Serotyping and antimicrobial resistance profile of Salmonella spp. isolated from crocodilians in captivity

Serotipificación y perfil de resistencia antimicrobiana de Salmonella spp. aislados de crocodilios en cautiverio

dc.creatorVargas-Abella, Julio César
dc.creatorTarazona-Manrique, Luis Edgar
dc.creatorAndrade-Becerra, Roy José
dc.date2023-01-15
dc.date.accessioned2023-09-06T21:40:39Z
dc.date.available2023-09-06T21:40:39Z
dc.identifierhttps://revistamvz.unicordoba.edu.co/article/view/3045
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8708394
dc.descriptionObjective. Determine the presence of Salmonella spp and the AMR in isolates of cloacal and muscle swabs in specimens of Caiman crocodilus fuscus and Crocodylus acutus in captivity, in Colombia. Materials and methods. A cross-sectional and descriptive sampling was carried out with a non-probabilistic design for convenience, once, during the month of February 2021. 150 Caiman crocodilus fuscus and Crocodylus acutus animals were included, with a 50/50 distribution. Cloacal and muscle samples were taken with sterile swabs. These were planted on MacConkey agar, then on SS agar. These positive samples were then reconfirmed on TSI agar. Serotyping was performed with specific monovalent and polyvalent antisera. The diffusion test was performed on Mueller-Hinton agar. Results. There was no growth of colonies in any muscle sample. The presence of the bacteria in cloacal samples of Caiman crocodilus fuscus was 36% and for Crocodylus acutus 50.6%. The serotypes with the highest detection percentage in both groups were serogroup C1 and polyvalent serogroup B. Some colonies were resistant to tetracycline, while other colonies had intermediate resistance to ampicillin, cefoxitin, sulfa trimethoprim, and tetracycline. Conclusions. This is the first report related to the detection of Salmonella spp. in alligator carcasses in the country, without finding the presence of the agent in any sample evaluated, however, the importance of continuing its monitoring is emphasized to guarantee innocuous productsen-US
dc.descriptionObjetivo. Determinar la presencia de Salmonella spp y el PRA en aislamientos de hisopados cloacales y musculares en especímenes de Caiman crocodilus fuscus y Crocodylus acutus en cautiverio en Colombia. Materiales y métodos. Se realizó un muestreo de tipo transversal y descriptivo con un diseño no probabilístico por conveniencia una única vez, durante el mes de febrero de 2021. Se incluyeron 150 animales de Caiman crocodilus fuscus y Crocodylus acutus, con una distribución 50/50. Se tomaron muestras cloacales y musculares con hisopos estériles. Estas se sembraron en agar MacConkey, posteriormente en agar SS. Luego, se reconfirmaron las muestras positivas en agar TSI. Se realizó la serotipifación con antisueros monovalentes y polivalentes específicos. Se realizó la prueba de difusión en agar Mueller-Hinton. Resultados. No existió crecimiento de colonias en ninguna muestra muscular. La presencia de la bacteria en muestras cloacales de Caiman crocodilus fuscus fue del 36% y para Crocodylus acutus de 50.6%, los dos serotipos con mayor porcentaje de detección en ambos grupos fueron el serogrupo C1 y el serogrupo polivalente B. Ocho colonias fueron resistentes a tetraciclina, mientras que otras colonias tuvieron una resistencia intermedia a ampicilina, cefoxitin, sulfatrimetropim y tetraciclina. Conclusiones. Este es el primer reporte relacionado con la detección de Salmonella spp. en canales de caimanes en el país, sin encontrar presencia del agente en ninguna muestra evaluada, sin embargo, se recalca la importancia de seguir su monitoreo para garantizar productos inocuos.es-ES
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dc.languagespa
dc.languageeng
dc.publisherUniversidad de Córdobaes-ES
dc.relationhttps://revistamvz.unicordoba.edu.co/article/view/3045/5216
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dc.relationhttps://revistamvz.unicordoba.edu.co/article/view/3045/5219
dc.relationhttps://revistamvz.unicordoba.edu.co/article/view/3045/5220
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dc.rightsDerechos de autor 2023 Julio César Vargas-Abella, Luis Edgar Tarazona-Manrique, Roy José Andrade-Becerraes-ES
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/4.0es-ES
dc.sourceJournal MVZ Cordoba; Vol. 28 No. 1 (2023): Revista MVZ Córdoba; 28(1) Enero-abril 2023; e3045en-US
dc.sourceRevista MVZ Córdoba; Vol. 28 Núm. 1 (2023): Revista MVZ Córdoba; 28(1) Enero-abril 2023; e3045es-ES
dc.source1909-0544
dc.source0122-0268
dc.source10.21897/rmvz.v28.n1.2023
dc.subjectreptilesen-US
dc.subjectantibiotic susceptibilityen-US
dc.subjectfoodborne diseasesen-US
dc.subjectmeat qualityen-US
dc.subjectreptileses-ES
dc.subjectSusceptibilidad antimicrobianaes-ES
dc.subjectenfermedades transmitidas por alimentoses-ES
dc.subjectcalidad de la carnees-ES
dc.titleSerotyping and antimicrobial resistance profile of Salmonella spp. isolated from crocodilians in captivityen-US
dc.titleSerotipificación y perfil de resistencia antimicrobiana de Salmonella spp. aislados de crocodilios en cautiverioes-ES
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion


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