Caracterización fenotípica y molecular de resistencia inducida por aplicaciones de silicio y biochar en etapa vegetativa de tomate (Solanum lycopersicum) haciafusarium oxysporium F. SP. Lycopersici raza 26202

dc.contributorCárdenas Hernández, Julián Fernando
dc.creatorSerna Maldonado, Christian Mateo
dc.date.accessioned2023-08-23T20:39:58Z
dc.date.accessioned2023-09-06T21:38:12Z
dc.date.available2023-08-23T20:39:58Z
dc.date.available2023-09-06T21:38:12Z
dc.date.created2023-08-23T20:39:58Z
dc.date.issued2017
dc.identifierSerna Maldonado, Christian Mateo. (2017). Caracterización fenotípica y molecular de resistencia inducida por aplicaciones de silicio y biochar en etapa vegetativa de tomate (solanum lycopersicum) haciafusarium oxysporium F. SP. Lycopersici raza 26202 [Trabajo de grado, Universidad de los Llanos]. Repositorio digital Universidad de los Llanos.
dc.identifierhttps://repositorio.unillanos.edu.co/handle/001/2969
dc.identifierUniversidad de los Llanos
dc.identifierRepositorio digital Universidad de los Llanos
dc.identifierhttps://repositorio.unillanos.edu.co/
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8708351
dc.description.abstractEste proyecto de investigacion fue realizado en las instalaciones del laboratorio IyerPascuzzi, perteneciente al departamento de botanica y fitopatologia de la Universidad de Purdue, West Lafayette. IN. USA. El trabajo busca alternativas de control de la enfermedad del suelo Fusarium oxisporium de tipo organico, practico, a bajo costo y efectivas para ser usadas por campesinos de la region del ariari y asi, brindar viabilidad fitosanitaria al cultivo del tomate a esta region de los Llanos Orientales de Colombia ya que las condiciones de edafoclimaticas son propicias para la proliferacion de esta enfermedad. La investigacion evaluo el efecto de la aplicación de enmiendas de Silicio y Biochar en plantas de tomate en estado vegetativo en el desarrollo de la enfermedad fúngica Fusarium oxysporium f. sp.lycopersici raza 26202. También hubo lugar a diversas pruebas de laboratorio enfocadas hacia el efecto de la aplicación de silicio a nivel fenotípico y molecular, donde se cuantifico y/o evaluó: acumulación de biomasa, acumulación de pigmentos (clorofila y antocianina) usando espectrofotómetro, colonización del patógeno a nivel vascular usando preservación de tejidos por técnica de parafina y posterior corte con micrótomo y expresión del gen de defensa PR-1 por electroforesis en gel.
dc.description.abstractThis research project was performed at the Iyer-Pascuzzi lab, which belongs to the botany and plant pathology department of Purdue University, West Lafayette. IN. USA. This research project aimed to use organic, practical, low cost and effective alternative strategies to control the soil borne pathogen Fusarium oxisporium. Thus, allowing farmers from the Ariari river basin cultivate tomato in this specific region of the oriental plains of Colombia which It´s soil/weather conditions allows this pathogen to thrive and spread. This research assessed the effect of Silicon and Biochar amendments on tomato plants in vegetative stage on the development of the soil borne disease Fusarium oxysporium f. sp.lycopersici strain 26202. The research also performed several laboratory trials emphasized on the effect of silicon amendments and It´s effect in a phenotypic and molecular level. Where It was quantified or assessed: biomass accumulation, pigments accumulation (chlorophyll and anthocyanin) using spectrophotometer, vascular colonization by the pathogen by paraffin preservation and later microscope analysis and gel electrophoresis for the defense gene PR-1.
dc.languagespa
dc.publisherUniversidad de los Llanos
dc.publisherFacultad de Ciencias Agropecuarias y Recursos Naturales
dc.publisherVillavicencio
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dc.titleCaracterización fenotípica y molecular de resistencia inducida por aplicaciones de silicio y biochar en etapa vegetativa de tomate (Solanum lycopersicum) haciafusarium oxysporium F. SP. Lycopersici raza 26202
dc.typeTrabajo de grado - Pregrado


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