Efectos letales y subletales del glifosato (roundup®) sobre larvas de dendropsophus colombianus (anura: hylidae)

dc.contributorRamírez Castaño, Viviana Andrea
dc.contributorMontaño Campaz, Milton Leoncio
dc.contributorGrupo de Ecología y Diversidad de Anfibios y Reptiles (Categoría B)
dc.creatorMoreno-M, Carlos Alberto
dc.date2022-05-02T18:15:47Z
dc.date2023-11-10
dc.date2022-05-02T18:15:47Z
dc.date2022-04-27
dc.date.accessioned2023-09-06T18:36:35Z
dc.date.available2023-09-06T18:36:35Z
dc.identifierhttps://repositorio.ucaldas.edu.co/handle/ucaldas/17583
dc.identifierUniversidad de Caldas
dc.identifierRepositorio de la Universidad de Caldas
dc.identifierhttps://repositorio.ucaldas.edu.co
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8699945
dc.descriptionIlustraciones, gráficas
dc.descriptionspa:El uso de los herbicidas como el glifosato en cualquiera de sus marcas comerciales ha sido un tema de controversia en las últimas décadas. Ha sido usado ampliamente en la agricultura para la erradicación de malezas y en el programa de aspersión aérea para erradicación de cultivos ilícitos hasta el año 2015. Diversos estudios han revelado efectos letales y subletales en anfibios, en su mayoría en renacuajos. Se evaluó la toxicidad aguda y crónica del glifosato en larvas de Dendropsophus columbianus, donde se encontró un efecto en la medidas morfométricas de la cabeza y en el desempeño locomotor. Se considera que el efecto del glifosato en D. columbianus es moderadamente tóxico.
dc.descriptioneng:The use of herbicides such as glyphosate in any of its commercial brands has been a subject of controversy in recent decades. This herbicide has been widely used by farmers for weed eradication and from 2000 to 2015 in the aerial spraying program for the eradication of illicit crops. Although their half-life in water is shorter than that in soil, amphibians are susceptible to this type of contaminant due to their dual life cycle. There have been a number of studies on the lethal and sublethal effects of glyphosate on various species around the world, with acute and chronic toxicity tests revealing a wide variety of results as measured by the median lethal dose and some body measurements. Evaluating these lethal effects with the median lethal dose (LC50) and sublethal effects on mormometric measurements and locomotor performance of glyphosate in its Roundup® commercial presentation on Dendropsophus colombianus larvae was the objective of this study. Adults of D. columbianus were collected and placed in fish tanks to induce an amplexus and subsequent fertilization of the clutch, waiting until the 25th larval stage to start the experiments. The LC50 was found to be 4mg/L over a 96h exposure period. The chronic tests revealed there was an effect of the sublethal doses LC01 and LC10 on the measures of head width and weight of the tadpoles, also with geometric morphometry variations in the shape and size of the head were revealed. Finally, the herbicide had an effect on locomotor performance where it directly affected exploration rate and average freezing time. Thanks to these findings, he considers that glyphosate is moderately toxic in this type of organisms.
dc.descriptionResumen/ Abstract/ Capítulo 1/ 1. Introducción/ 2. Metodología/ 2.1 Obtención de larvas y aclimatación/ 2.2 Ensayo de toxicidad aguda/ 2.3 Ensayo de toxicidad crónica/ 2.3.1 Medidas morfométricas lineales/ 2.3.1 Desempeño locomotor/ 2.3.1 Morfometría geométrica/ 2.4 Análisis de datos/ 3. Resultados/ 3.1 Toxicidad aguda/ 3.2 Toxicidad crónica/ 3.2.1 Medidas morfo métricas lineales/ 3.2.1 Desempeño locomotor/ 3.2.1 Morfometría geométrica/ 4. Discusión/ 5. Conclusiones/ 6. Bibliografía.
dc.descriptionUniversitario
dc.descriptionSe realizará publicación científica (artículo, ponencia, otro)
dc.descriptionBiólogo(a)
dc.descriptionEcotoxicología
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.formatapplication/pdf
dc.languageeng
dc.languagespa
dc.publisherFacultad de Ciencias Exactas y Naturales
dc.publisherManizales
dc.publisherBiología
dc.relationAnnett, R., Habibi, H. R., & Hontela, A. (2014). Impact of glyphosate and glyphosate‐based herbicides on the freshwater environment. Journal of Applied Toxicology, 34(5), 458- 479.
dc.relationArendt, J. (2010). Morphological correlates of sprint swimming speed in five species of spadefoot toad tadpoles: comparison of morphometric methods. Journal of Morphology, 271(9), 1044-1052.
dc.relationBernal, M. H., Solomon, K. R., & Carrasquilla, G. (2009). Toxicity of formulated glyphosate (Glyphos) and Cosmo-Flux to larval Colombian frogs 1. Laboratory acute toxicity. Journal of Toxicology and Environmental Health, Part A, 72(15-16), 961-965.
dc.relationBolívar, W., Renjifo, J.M. (2004): Dendropsophus columbianus. IUCN Red List of Threatened Species. 2018; Version 2019.1. Disponible en: www.iucnredlist.org. Accessed on 25 March 2019.
dc.relationBrain, R. A., & Solomon, K. R. (2009). Comparison of the hazards posed to amphibians by the glyphosate spray control program versus the chemical and physical activities of coca production in Colombia. Journal of Toxicology and Environmental Health, Part A, 72(15-16), 937-948.
dc.relationBridges, C. M. (1997). Tadpole swimming performance and activity affected by acute exposure to sublethal levels of carbaryl. Environmental Toxicology and Chemistry, 16(9), 1935-1939.
dc.relationCosta, R. N., Solé, M., & Nomura, F. (2017). Agropastoral activities increase fluctuating asymmetry in tadpoles of two neotropical anuran species. Austral Ecology, 42(7), 801-809.
dc.relationDavalos, L. M., Bejarano, A. C., Hall, M. A., Correa, H. L., Corthals, A., & Espejo, O. J. (2011). Forests and drugs: Coca-driven deforestation in tropical biodiversity hotspots. Environmental science & technology, 45(4), 1219-1227.
dc.relationDenoël, M., Libon, S., Kestemont, P., Brasseur, C., Focant, J. F., & De Pauw, E. (2013). Effects of a sublethal pesticide exposure on locomotor behavior: a video-tracking analysis in larval amphibians. Chemosphere, 90(3), 945-951.
dc.relationDuellman, W. E., & Trueb, L. (1983). Frogs of the Hyla columbiana group: taxonomy and phylogenetic relationships. Advances in herpetology and evolutionary biology, 33- 51.
dc.relationDuke, S. O., & Powles, S. B. (2008). Glyphosate: a once‐in‐a‐century herbicide. Pest management science, 64(4), 319-325.
dc.relationFrost, D. R. 2019. Amphibian Species of the World: an Online Reference. Version 6.0 (Date of access). Electronic Database accessible at http://research.amnh.org/herpetology/amphibia/index.html. American Museum of Natural History, New York, USA. Accesed on 25 March 2019.
dc.relationFigueiredo, J., & de Jesus Rodrigues, D. (2014). Effects of four types of pesticides on survival, time and size to metamorphosis of two species of tadpoles (Rhinella and Physalaemus centralis) from the southern Amazon, Brazil. The Herpetological Journal, 24(1), 7-15.
dc.relationFort, D. J., Degitz, S., Tietge, J., & Touart, L. W. (2007). The hypothalamic-pituitary-thyroid (HPT) axis in frogs and its role in frog development and reproduction. Critical reviews in toxicology, 37(1-2), 117-161
dc.relationGiesy, J. P., Dobson, S., & Solomon, K. R. (2000). Ecotoxicological risk assessment for Roundup® herbicide. Reviews of environmental contamination and toxicology, 35- 120.
dc.relationGosner, K. L. (1960). A simplified table for staging anuran embryos and larvae with notes on identification. Herpetologica, 16(3), 183-190.
dc.relationHenao Muñoz, L. M., Montes Rojas, C. M., & Bernal Bautista, M. H. (2015). Acute toxicity and sublethal effects of the mixture glyphosate (Roundup® Active) and CosmoFlux® 411F to anuran embryos and tadpoles of four Colombian species. Revista de biologia tropical, 63(1), 223-233.
dc.relationHerek, J. S., Vargas, L., Trindade, S. A. R., Rutkoski, C. F., Macagnan, N., Hartmann, P. A., & Hartmann, M. T. (2020). Can environmental concentrations of glyphosate affect 21 survival and cause malformation in amphibians? Effects from a glyphosate-based herbicide on Physalaemus cuvieri and P. gracilis (Anura: Leptodactylidae). Environmental Science and Pollution Research, 27(18), 22619- 22630.
dc.relationHerkovits, J., & Perez-Coll, C. S. (2003). AMPHITOX: a customized set of toxicity tests employing amphibian embryos. In Multiple stressor effects in relation to declining amphibian populations. ASTM International.
dc.relationHoyos-Hoyos, J. M., Isaacs-Cubides, P., Devia, N., Galindo-Uribe, D. M., & Acosta-Galvis, A. R. (2012). An approach to the ecology of the herpetofauna in agroecosystems of the Colombian coffee zone. South American Journal of Herpetology, 7(1), 25-35.
dc.relationHowe, C. M., Berrill, M., Pauli, B. D., Helbing, C. C., Werry, K., & Veldhoen, N. (2004). Toxicity of glyphosate‐based pesticides to four North American frog species. Environmental Toxicology and Chemistry, 23(8), 1928-1938.
dc.relationJones, D. K., Hammond, J. I., & Relyea, R. A. (2011). Competitive stress can make the herbicide Roundup® more deadly to larval amphibians. Environmental Toxicology and Chemistry, 30(2), 446-454
dc.relationKatzenberger, M., Hammond, J., Duarte, H., Tejedo, M., Calabuig, C., & Relyea, R. A. (2014). Swimming with predators and pesticides: how environmental stressors affect the thermal physiology of tadpoles. PloS one, 9(5), e98265
dc.relationJayawardena, U. A., Rajakaruna, R. S., Navaratne, A. N., & Amerasinghe, P. H. (2010). Toxicity of agrochemicals to common hourglass tree frog (Polypedates cruciger) in acute and chronic exposure. International Journal of Agriculture and Biology, 12(5), 641-648.
dc.relationLajmanovich, R., Lorenzatti, E., Maitre, M. I., Enrique, S., & Peltzer, P. (2003). Comparative acute toxicity of the commercial herbicides glyphosate to neotropical tadpoles Scinax nasicus (Anura: Hylidae). Fresenius Environmental Bulletin, 12(4), 364-367.
dc.relationLanctot, C., Navarro-Martín, L., Robertson, C., Park, B., Jackman, P., Pauli, B. D., & Trudeau, V. L. (2014). Effects of glyphosate-based herbicides on survival, development, growth and sex ratios of wood frog (Lithobates sylvaticus) tadpoles. II: agriculturally relevant exposures to Roundup WeatherMax® and Vision® under laboratory conditions. Aquatic Toxicology, 154, 291-303.
dc.relationMeza‐Joya, F. L., Ramírez‐Pinilla, M. P., & Fuentes-Lorenzo, J. L. (2013). Toxic, cytotoxic, and genotoxic effects of a glyphosate formulation (Roundup® SL–Cosmoflux® 411F) in the direct‐developing frog Eleutherodactylus johnstonei. Environmental and Molecular Mutagenesis, 54(5), 362-373.
dc.relationPavan, F. A., Samojeden, C. G., Rutkoski, C. F., Folador, A., Da Fré, S. P., Müller, C., ... & Hartmann, M. T. (2021). Morphological, behavioral and genotoxic effects of glyphosate and 2, 4-D mixture in tadpoles of two native species of South American amphibians. Environmental Toxicology and Pharmacology, 85, 103637.
dc.relationRamírez-Chaves, H. E., Pisso-Florez, G. A., Liévano-Bonilla, A. F., Ayerbe-Quiñones, F., Criollo, M. A., & Noguera-Urbano, E. A. (2018). On the distribution of the endemic Boettger’s Colombian Treefrog, Dendropsophus columbianus (Anura: Hylidae) with distribution extension in southwestern Colombia. Herpetology Notes, 11, 49-58.
dc.relationRelyea, R. A. (2004). Growth and survival of five amphibian species exposed to combinations of pesticides. Environmental Toxicology and Chemistry: An International Journal, 23(7), 1737-1742
dc.relationRelyea, R. A. (2005). The lethal impact of Roundup on aquatic and terrestrial amphibians. Ecological applications, 15(4), 1118-1124
dc.relationRelyea, R. A., & Jones, D. K. (2009). The toxicity of Roundup Original Max® to 13 species of larval amphibians. Environmental Toxicology and Chemistry: An International Journal, 28(9), 2004-2008.
dc.relationRodriguez, A., Zhang, H., Klaminder, J., Brodin, T., Andersson, P. L., & Andersson, M. (2018). ToxTrac: a fast and robust software for tracking organisms. Methods in Ecology and Evolution, 9(3), 460-464.
dc.relationSalgado-Negret, B.A. (Ed.). (2016). La ecología funcional como aproximación al estudio, manejo y conservación de la biodiversidad: protocolos y aplicaciones. Instituto de Investigación de Recursos Biológicos Alexander von Humboldt.
dc.relationSchönbrunn, E., Eschenburg, S., Shuttleworth, W. A., Schloss, J. V., Amrhein, N., Evans, J. N., & Kabsch, W. (2001). Interaction of the herbicide glyphosate with its target enzyme 5-enolpyruvylshikimate 3-phosphate synthase in atomic detail. Proceedings of the National Academy of Sciences, 98(4), 1376-1380
dc.relationSievers, M., Hale, R., Parris, K. M., Melvin, S. D., Lanctot, C. M., & Swearer, S. E. (2019). Contaminant-induced behavioural changes in amphibians: A meta-analysis. Science of the Total Environment, 693, 133570.
dc.relationSparling, D. W. (2017). Basics of Ecotoxicology. CRC Press.
dc.relationSolomon, K. R., Anadón, A., Carrasquilla, G., Cerdeira, A. L., Marshall, E. J. P., & Sanin, L. H. (2007). Coca and poppy eradication in Colombia: Environmental and human health assessment of aerially applied glyphosate. In Reviews of environmental contamination and toxicology (pp. 43-125). Springer, New York, NY.
dc.relationWagner, N., Reichenbecher, W., Teichmann, H., Tappeser, B., & Lötters, S. (2013). Questions concerning the potential impact of glyphosate‐based herbicides on amphibians. Environmental Toxicology and Chemistry, 32(8), 1688-1700.
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.rightshttp://purl.org/coar/access_right/c_14cb
dc.subjectHerbicidas
dc.subjectToxicidad
dc.subjectGlifosato
dc.subjectEfectos letales y subletales
dc.subjectDosis letal media
dc.subjectMorfometría geométrica
dc.subjectDesempeño locomotor
dc.titleEfectos letales y subletales del glifosato (roundup®) sobre larvas de dendropsophus colombianus (anura: hylidae)
dc.typeTrabajo de grado - Pregrado
dc.typehttp://purl.org/coar/resource_type/c_7a1f
dc.typeText
dc.typeinfo:eu-repo/semantics/bachelorThesis
dc.typehttps://purl.org/redcol/resource_type/TP
dc.typeinfo:eu-repo/semantics/publishedVersion


Este ítem pertenece a la siguiente institución