Desarrollo de un protocolo estandarizado y repetible para obtener plasma altamente concentrado rico en plaquetas en caballos.

Development of a standardized and repeatable protocol to obtain highly concentrated platelet-rich plasma in horses.

dc.creatorCuevas Ramos, Gabriel
dc.creatorArguelles, David
dc.creatorVidal, Luis
dc.creatorPrades, Marta
dc.date2019-07-01 00:00:00
dc.date2020-12-09T16:52:37Z
dc.date2019-07-01 00:00:00
dc.date2020-12-09T16:52:37Z
dc.date2019-07-01
dc.date.accessioned2023-09-06T18:31:23Z
dc.date.available2023-09-06T18:31:23Z
dc.identifierhttps://doi.org/10.17151/vetzo.2019.13.2.5
dc.identifierhttps://repositorio.ucaldas.edu.co/handle/ucaldas/13651
dc.identifier10.17151/vetzo.2019.13.2.5
dc.identifier2011-5415
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8698666
dc.descriptionIntroducción: el plasma rico en plaquetas (PRP) es un producto autólogo derivado de la sangre con varias aplicaciones terapéuticas. Se ha demostrado que los factores de crecimiento plaquetario estimulan la curación y reducen el dolor. Su uso en la industria equina, particularmente para problemas ortopédicos como la osteoartritis o la tendinitis ha ido en aumento. Sin embargo, el PRP carece de reproducibilidad y a veces, de calidad, ya que no solo los factores individuales (raza, edad, sexo) y ambientales (hora del día, estado de hidratación), sino también los métodos de laboratorio pueden afectar su calidad final. Objetivos: nuestro objetivo fue desarrollar y normalizar un protocolo para la preparación de PRP, que será repetible para uso equino, a través de un kit desechable estéril con una centrífuga estándar, que minimiza las variantes de laboratorio. Métodos y resultados: se estableció el protocolo final que resultó en un PRP con una concentración de plaquetas muy alta (6,4±1,3 veces mayor) y recuentos bajos de glóbulos blancos (3,7±1,8 x106/ml). El PRP final tenía concentraciones de factor de crecimiento derivado de plaquetas con un promedio de 37 ng/ml. Conclusión: El protocolo es altamente repetible y simple de realizar, y el kit es adecuado para usar en condiciones de campo porque no permite el contacto entre la muestra y el aire ambiente a lo largo del proceso.
dc.descriptionIntroduction: Platelet-rich plasma (PRP) is an autologous blood-derived product with several therapeutic applications. Platelet growth factors have been shown to stimulate healing and reduce pain. Its use in the equine industry, particularly for orthopedic problems such as osteoarthritis or tendonitis has been increasing. However, PRP lacks reproducibility and, sometimes, quality since not only individual (breed, age, gender) and environmental (time of the day, hydration status) factors, but also laboratory methods can affect its final quality. Aims: we aimed to develop and normalize a protocol for PRP preparation, which will be repeatable for equine use, through a sterile disposable kit with a standard centrifuge, which minimizes laboratory variants. Methods and results: The final protocol resulting in a PRP with very high platelet concentration (6.4 ± 1.3 fold increase) and low white blood cell counts (3.7 ± 1.8 x106/mL) was stablished. The usual fold increase reported is 2 to 3. The final PRP had concentrations of platelet-derived growth factor with an average of 37ng/ml. Conclusion: The protocol is highly repeatable and simple to perform and the kit is suitable to use under field conditions because it does not permit contact between the sample and ambient air along the process.
dc.formatapplication/pdf
dc.languageeng
dc.publisherUniversidad de Caldas
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dc.relationNúm. 2 , Año 2019 : Julio - Diciembre
dc.relationhttps://revistasojs.ucaldas.edu.co/index.php/vetzootec/article/download/347/276
dc.rightsDerechos de autor 2019 David Arguelles
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/4.0
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightsEsta obra está bajo licencia internacional Creative Commons Reconocimiento-NoComercial-CompartirIgual 4.0.
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.sourcehttps://revistasojs.ucaldas.edu.co/index.php/vetzootec/article/view/347
dc.subjectplatelet-rich plasma
dc.subjecthorses
dc.subjectnew method
dc.subjectplasma rico en plaquetas
dc.subjectcaballos
dc.subjectnuevo método
dc.titleDesarrollo de un protocolo estandarizado y repetible para obtener plasma altamente concentrado rico en plaquetas en caballos.
dc.titleDevelopment of a standardized and repeatable protocol to obtain highly concentrated platelet-rich plasma in horses.
dc.typeSección Research / investigación
dc.typeArtículo de revista
dc.typeJournal Article
dc.typehttp://purl.org/coar/resource_type/c_6501
dc.typeText
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typehttp://purl.org/coar/version/c_970fb48d4fbd8a85


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