Estimación de parámetros en modelo biomatemáticos: producción de ácido itacónico

dc.contributorMatallana Pérez, Luis Gerónimo
dc.creatorSánchez Rendón, Julio César
dc.date2021-05-19T20:55:05Z
dc.date2021-05-19T20:55:05Z
dc.date2021-05-17
dc.date.accessioned2023-09-06T18:25:24Z
dc.date.available2023-09-06T18:25:24Z
dc.identifierhttps://repositorio.ucaldas.edu.co/handle/ucaldas/16620
dc.identifierUniversidad de Caldas
dc.identifierRepositorio institucional Universidad de Caldas
dc.identifierhttps://repositorio.ucaldas.edu.co
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8697098
dc.descriptionIlustraciones
dc.descriptionspa: El ácido itacónico es una molécula orgánica con un mercado de 40,000 toneladas anuales y amplia variedad de usos industriales como aditivo de detergentes, productos farmacéuticos, herbicidas, removedor de resinas, compuestos dentales, entre otros. Esta sustancia se produce generalmente por fermentación del hongo filamentoso Aspergillus terreus, sin embargo, no se han propuesto modelos matemáticos detallados del comportamiento dinámico de los metabolitos involucrados en su producción. El presente trabajo muestra el desarrollo de un modelo matemático basado en red metabólica para la producción de ácido itacónico, con diferenciación entre compartimientos celulares y la incorporación del antiporte oxalacetato/cis-aconitato presente en la mitocondria. Los parámetros de este modelo matemático fueron calculados a partir del optimizador enjambre de partículas (Global optimization toolbox) y el integrador “ode15s” ambos presentes en Matlab® R2018, función objetivo de mínimos cuadrados y los datos experimentales obtenidos por Pretruccioli y Rychtera & Wase. El modelo presenta un ajuste considerable (R2 = 0.6819) para el mejor conjunto de datos. Así mismo, el modelo propuesto es capaz de describir la dinámica del proceso de fermentación de ácido itacónico a nivel de citosol y mitocondria, el cual no ha sido previamente reportado.
dc.descriptioneng: Itaconic acid is an organic molecule with a market of 40,000 tons per year and a wide variety of industrial uses such as detergent additive, pharmaceuticals, herbicides, resin remover, dental compounds, among others. This substance is generally produced by fermentation of the filamentous fungus Aspergillus terreus; however, detailed mathematical models of the dynamic behavior of the metabolites involved in its production have not been proposed. The present work shows the development of a mathematical model based on a metabolic network for itaconic acid production, with differentiation between cellular compartments and the incorporation of the oxaloacetate/cis-aconitate antiport present in the mitochondria. The parameters of this mathematical model were calculated with particle swarm optimizer (Global optimization toolbox) and the integrator "ode15s" both present in Matlab® R2018, least squares objective function and the experimental data obtained by Pretruccioli and Rychtera & Wase. The model presents a considerable fit (R2 = 0.6819) for the best data set. Likewise, the proposed model is able to describe the dynamics of the itaconic acid fermentation process at the cytosol and mitochondrial level, which has not been previously reported.
dc.descriptionIntroducción / Materiales y métodos / Producción bioquímica de ácido itacónico / Comportamiento cualitativo de la fermentación de ácido itacónico / Estructuración del modelo matemático / Metabolismo involucrado en la producción de ácido itacónico / Ecuaciones de transporte / Reacciones bioquímicas / Modelo dinámico de producción de ácido itacónico / Algoritmo de optimización / Problema de estimación de parámetros / Resultados / Discusión Conclusiones y recomendaciones / Conclusiones / Recomendaciones / Referencias bibliográficas
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dc.languageeng
dc.languagespa
dc.publisherManizales
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dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectácido itacónico
dc.subjectmodelo matemático
dc.subjectestimación de parámetros
dc.subjectitaconic acid
dc.subjectmathematical model
dc.subjectparameter estimation
dc.subjectBiología molecular
dc.subjectBioquímica
dc.titleEstimación de parámetros en modelo biomatemáticos: producción de ácido itacónico
dc.typeSimulación
dc.typeTrabajo de grado - Pregrado
dc.typehttp://purl.org/coar/resource_type/c_7a1f
dc.typeText
dc.typeinfo:eu-repo/semantics/bachelorThesis
dc.typeinfo:eu-repo/semantics/publishedVersion


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