Aislamiento y caracterización molecular de micobacterias no tuberculosas en el sistema de distribución de agua en un hospital de Lima (Perú).

Isolation and molecular characterization of nontuberculous mycobacteria in the water distribution system in a hospital of Lima (Perú).

dc.creatorCabello Vílchez, Alfonso Martín
dc.creatorNúñez Ato, Ronald G.
dc.date2018-07-01 00:00:00
dc.date2020-12-09T16:48:55Z
dc.date2018-07-01 00:00:00
dc.date2020-12-09T16:48:55Z
dc.date2018-07-01
dc.date.accessioned2023-09-06T18:15:54Z
dc.date.available2023-09-06T18:15:54Z
dc.identifier1657-9550
dc.identifierhttps://doi.org/10.17151/biosa.2018.17.2.1
dc.identifierhttps://repositorio.ucaldas.edu.co/handle/ucaldas/12752
dc.identifier10.17151/biosa.2018.17.2.1
dc.identifier2462-960X
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8694382
dc.descriptionIntroducción: Las infecciones por micobacterias pueden causar lesiones cutáneas y pulmonares; las micobacterias pueden hallarse en fuentes de agua intrahospitalarias, pudiendo llegar a los pacientes inmunosuprimidos y provocar enfermedades severas. Objetivo: Aislar y caracterizar micobacterias no tuberculosas en el sistema de distribución de agua en un hospital de Lima. Materiales y métodos: Se aislaron las micobacterias en medios convencionales a base de huevo; y se emplearon pruebas bioquímicas y herramientas moleculares como la reacción en cadena de la polimerasa ―PCR― y secuenciación para su identificación. Para el análisis de secuenciación se empleó el GenBank y para el árbol filogenético se utilizó MEGA 6. Resultados: Fue posible el aislamiento de bacilos ácido alcohol resistentes en muestras de agua potable fría, aunque no en agua caliente del hospital. Se aislaron e identificaron varias especies de micobacterias no tuberculosas en las muestras de agua, siendo el 100 % de crecimiento rápido, estas fueron: Mycobacterium canariasense; M. frederiksbergense; M. mucogenicum; Mycobacterium fortuitum y Mycobacterium chelonae. Conclusiones: Esta es la primera vez que se aíslan micobacterias en agua potable de un hospital en Perú. Se concluye que la existencia de micobacterias en el agua del hospital podría ser fuente potencial de brotes nosocomiales, por lo que debería tenerse en cuenta su estudio periódicamente.
dc.descriptionIntroduction: Mycobacterial infections can cause skin and lung lesions. Mycobacteria can be found in intra-hospital water sources which can reach immuno-suppressed patients and cause severe disease. Objective: To isolate and characterize non-tuberculous mycobacteria in the water distribution system in a hospital in Lima. Materials and Methods: Mycobacteria were isolated in conventional culture egg-based media and biochemical tests and molecular tools such as polymerase chain reaction (PCR) and sequencing were used for their identification. The GenBank was used for sequencing analysis and MEGA 6 was used for the phylogenetic tree. Results: Isolation of acid-fast bacilli was possible in samples of cold drinking water, although not in the hospital hot water. Several species of non-tuberculous mycobacteria were isolated and identified in the water samples being a 100% of rapid growth such as Mycobacterium canariasense, M. frederiksbergense, Mycobacterium fortuitum, Mycobacterium chelonae, and M. mucogenicum. Conclusions: This is the first time that mycobacteria are isolated in drinking water at a hospital in Perú. It is concluded that the existence of mycobacteria in hospital water could be a potential source of nosocomial outbreaks and should be analyzed periodically.
dc.formatapplication/pdf
dc.languagespa
dc.publisherUniversidad de Caldas
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dc.relationNúm. 2 , Año 2018 : Julio - Diciembre
dc.relationhttps://revistasojs.ucaldas.edu.co/index.php/biosalud/article/download/11/4
dc.rightsDerechos de autor 2018 Alfonso Martín Cabello Vílchez
dc.rightshttps://creativecommons.org/licenses/by-nc-sa/4.0
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightsEsta obra está bajo licencia internacional Creative Commons Reconocimiento-NoComercial-CompartirIgual 4.0.
dc.rightshttp://purl.org/coar/access_right/c_abf2
dc.sourcehttps://revistasojs.ucaldas.edu.co/index.php/biosalud/article/view/11
dc.subjectatypical mycobacteria
dc.subjectsoil and water mycobacterium
dc.subjectnon-tuberculous mycobacteria
dc.subjectmicobacterias atípicas
dc.subjectmicobacterias de tierra y agua
dc.subjectmicobacterias no tuberculosas
dc.titleAislamiento y caracterización molecular de micobacterias no tuberculosas en el sistema de distribución de agua en un hospital de Lima (Perú).
dc.titleIsolation and molecular characterization of nontuberculous mycobacteria in the water distribution system in a hospital of Lima (Perú).
dc.typeSección Artículos Originales
dc.typeArtículo de revista
dc.typeJournal Article
dc.typehttp://purl.org/coar/resource_type/c_6501
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dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typehttp://purl.org/coar/version/c_970fb48d4fbd8a85


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