Otro
Susceptibility of Candida albicans and Candida dubliniensis to erythrosine- and LED-mediated photodynamic therapy
Registro en:
Archives of Oral Biology. Oxford: Pergamon-Elsevier B.V. Ltd, v. 56, n. 11, p. 1299-1305, 2011.
0003-9969
10.1016/j.archoralbio.2011.05.013
WOS:000297033700014
WOS000297033700014.pdf
Autor
Borges Pereira Costa, Anna Carolina
de Campos Rasteiro, Vanessa Maria
Pereira, Cristiane Aparecida
Hashimoto, Emily Setsuko Halter da Silva
Beltrame Junior, Milton
Junqueira, Juliana Campos
Cardoso Jorge, Antonio Olauo
Resumen
The effect of erythrosine- and LED-mediated photodynamic therapy (PDT) on planktonic cultures and biofilms of Candida albicans and Candida dubliniensis was evaluated. Planktonic cultures of standardized suspensions (10(6) cells/mL) of C. albicans and C. dubliniensis were treated with erythrosine concentrations of 0.39-200 mu M and LEDs in a 96-well microtiter plate. Biofilms formed by C. albicans and C. dubliniensis in the bottom of a 96-well microtiter plate were treated with 400 mu M erythrosine and LEDs. After PDT, the biofilms were analysed by scanning electron microscopy (SEM). The antimicrobial effect of PDT against planktonic cultures and biofilms was verified by counting colony-forming units (CFU/mL), and the data were submitted to analysis of variance and the Tukey test (P < 0.05). C. albicans and C. dubliniensis were not detectable after PDT of planktonic cultures with erythrosine concentrations of 3.12 mu M or higher. The CFU/mL values obtained from biofilms were reduced 0.74 log(10) for C. albicans and 0.21 log(10) for C. dubliniensis. SEM revealed a decrease in the quantity of yeasts and hyphae in the biofilm after PDT. In conclusion, C. albicans and C. dubliniensis were susceptible to erythrosine- and LED-mediated PDT, but the biofilms of both Candida species were more resistant than their planktonic counterparts. (C) 2011 Elsevier Ltd. All rights reserved. Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)