High Prevalence of bla(CTX-M) Extended Spectrum Beta-Lactamase Genes in Klebsiella pneumoniae Isolates from a Tertiary Care Hospital: First report of bla(SHV-12), bla(SHV-31), bla(SHV-38), and bla(CTX-M-15) in Brazil

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorTollentino, Fernanda M.
dc.creatorPolotto, Milena
dc.creatorNogueira, Mauricio L.
dc.creatorLincopan, Nilton
dc.creatorNeves, Patricia
dc.creatorMamizuka, Elsa M.
dc.creatorRemeli, Gisele A.
dc.creatorDe Almeida, Margarete T. G.
dc.creatorRubio, Fernando G.
dc.creatorNogueira, Mara C. L.
dc.date2014-05-20T14:01:16Z
dc.date2014-05-20T14:01:16Z
dc.date2011-03-01
dc.date.accessioned2017-04-05T21:25:07Z
dc.date.available2017-04-05T21:25:07Z
dc.identifierMicrobial Drug Resistance. New Rochelle: Mary Ann Liebert Inc., v. 17, n. 1, p. 7-16, 2011.
dc.identifier1076-6294
dc.identifierhttp://hdl.handle.net/11449/21650
dc.identifier10.1089/mdr.2010.0055
dc.identifierWOS:000288073500002
dc.identifierWOS000288073500002.pdf
dc.identifierhttp://dx.doi.org/10.1089/mdr.2010.0055
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/867193
dc.descriptionThe aim of this study was to investigate the presence and prevalence of bla(TEM), bla(SHV), and bla(CTX-M) and bla(GES)-like genes, responsible for extended spectrum beta-lactamases (ESBLs) production in clinical isolates of Klebsiella pneumoniae collected from a Brazilian tertiary care hospital. Sixty-five ESBL producing K. pneumoniae isolates, collected between 2005 and 2007, were screened by polymerase chain reaction (PCR). Identification of bla genes was achieved by sequencing. Genotyping of ESBL producing K. pneumoniae was performed by the enterobacterial repetitive intergenic consensus-PCR with cluster analysis by the Dice coefficient. The presence of genes encoding ESBLs was confirmed in 59/65 (90.8%) isolates, comprising 20 bla(CTX-M-2), 14 bla(CTX-M-59), 12 bla(CTX-M-15), 9 bla(SHV-12), 1 bla(SHV-2), 1 bla(SHV-2a), 1 bla(SHV-5), and 1 bla(SHV-31) genes. The ESBL genes bla(SHV-12), bla(SHV-31), and bla(CTX-M-15), and the chromosome-encoded SHV-type beta-lactamase capable of hydrolyzing imipenem were detected in Brazil for the first time. The analysis of the enterobacterial repetitive intergenic consensus-PCR band patterns revealed a high rate of multiclonal bla(CTX-M) carrying K. pneumoniae isolates (70.8%), suggesting that dissemination of encoding plasmids is likely to be the major cause of the high prevalence of these genes among the K. pneumoniae isolates considered in this study.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.languageeng
dc.publisherMary Ann Liebert, Inc.
dc.relationMicrobial Drug Resistance
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.titleHigh Prevalence of bla(CTX-M) Extended Spectrum Beta-Lactamase Genes in Klebsiella pneumoniae Isolates from a Tertiary Care Hospital: First report of bla(SHV-12), bla(SHV-31), bla(SHV-38), and bla(CTX-M-15) in Brazil
dc.typeOtro


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