Annexin 1 mimetic peptide protects against renal ischemia/reperfusion injury in rats

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorFacio, Fernando N.
dc.creatorSena, Angela A.
dc.creatorAraujo, Leandro P.
dc.creatorMendes, Gloria E.
dc.creatorCastro, Isac
dc.creatorLuz, Marcus A. M.
dc.creatorYu, Luis
dc.creatorOliani, Sonia Maria
dc.creatorBurdmann, Emmanuel A.
dc.date2014-05-20T14:01:10Z
dc.date2016-10-25T17:08:28Z
dc.date2014-05-20T14:01:10Z
dc.date2016-10-25T17:08:28Z
dc.date2011-01-01
dc.date.accessioned2017-04-05T21:24:55Z
dc.date.available2017-04-05T21:24:55Z
dc.identifierJournal of Molecular Medicine-jmm. New York: Springer, v. 89, n. 1, p. 51-63, 2011.
dc.identifier0946-2716
dc.identifierhttp://hdl.handle.net/11449/21617
dc.identifierhttp://acervodigital.unesp.br/handle/11449/21617
dc.identifier10.1007/s00109-010-0684-4
dc.identifierWOS:000288363200007
dc.identifierhttp://dx.doi.org/10.1007/s00109-010-0684-4
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/867167
dc.descriptionInflammation is currently recognized as a key mechanism in the pathogenesis of renal ischemia-reperfusion (I/R) injury. The importance of infiltrating neutrophil, lymphocytes, and macrophage in this kind of injury has been assessed with conflicting results. Annexin 1 is a protein with potent neutrophil anti-migratory activity. In order to evaluate the effects of annexin A1 on renal I/R injury, uninephrectomized rats received annexin A1 mimetic peptide Ac2-26 (100 mu g) or vehicle before 30 min of renal artery clamping and were compared to sham surgery animals. Annexin A1 mimetic peptide granted a remarkable protection against I/R injury, preventing glomerular filtration rate and urinary osmolality decreases and acute tubular necrosis development. Annexin A1 infusion aborted neutrophil extravasation and attenuated macrophage infiltration but did not prevent tissue lymphocyte traffic. I/R increased annexin A1 expression (assessed by transmission electron microscopy) in renal epithelial cells, which was attenuated by exogenous annexin A1 infusion. Additionally, annexin A1 reduced I/R injury in isolated proximal tubules suspension. Annexin A1 protein afforded striking functional and structural protection against renal I/R. These results point to an important role of annexin A1 in the epithelial cells defense against I/R injury and indicate that neutrophils are key mediators for the development of tissue injury after renal I/R. If these results were confirmed in clinical studies, annexin A1 might emerge as an important tool to protect against I/R injury in renal transplantation and in vascular surgery.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageeng
dc.publisherSpringer
dc.relationJournal of Molecular Medicine-jmm
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectAnnexin A1
dc.subjectAcute kidney injury
dc.subjectIschemia/reperfusion injury
dc.subjectKidney
dc.subjectNeutrophils
dc.subjectAcute tubular necrosis
dc.titleAnnexin 1 mimetic peptide protects against renal ischemia/reperfusion injury in rats
dc.typeOtro


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