| dc.contributor | Universidade Estadual Paulista (UNESP) | |
| dc.creator | Pinto, M. E. | |
| dc.creator | Vilamaior, P. S. L. | |
| dc.creator | Taboga, S. R. | |
| dc.creator | Goes, R. M. | |
| dc.date | 2014-05-20T14:01:00Z | |
| dc.date | 2016-10-25T17:08:20Z | |
| dc.date | 2014-05-20T14:01:00Z | |
| dc.date | 2016-10-25T17:08:20Z | |
| dc.date | 2008-02-01 | |
| dc.date.accessioned | 2017-04-05T21:24:23Z | |
| dc.date.available | 2017-04-05T21:24:23Z | |
| dc.identifier | Tissue & Cell. Edinburgh: Churchill Livingstone, v. 40, n. 1, p. 31-42, 2008. | |
| dc.identifier | 0040-8166 | |
| dc.identifier | http://hdl.handle.net/11449/21550 | |
| dc.identifier | http://acervodigital.unesp.br/handle/11449/21550 | |
| dc.identifier | 10.1016/j.tice.2007.09.002 | |
| dc.identifier | WOS:000252695300004 | |
| dc.identifier | 0000-0002-0970-4288 | |
| dc.identifier | http://dx.doi.org/10.1016/j.tice.2007.09.002 | |
| dc.identifier.uri | http://repositorioslatinoamericanos.uchile.cl/handle/2250/867103 | |
| dc.description | Single high doses of estrogen (35 mg/kg body weight) were administered to young rats aiming to exacerbate its effects on germ cell populations. The short-term (1 week) and medium-term (7 weeks) consequences of this estrogenic treatment (ET) on the testis were evaluated using light and electron microscopies, quantitative methods and TUNEL reaction. Short-term ET led to 50% atrophy of the testis, however, in the medium term the gonado-somatic index was recovered. No histopathological alterations were found at seminiferous epithelium except for short-term severe degeneration of elongated spermatids (EL) and low frequency of these cells in both time intervals. Two morphologically distinct patterns of degeneration were observed: (1) clusters of EL which were TUNEL-negative and exhibited bizarre appearance and nuclear fragmentation, (2) isolated apoptotic EL within the cytoplasm of Sertoli cells (SC). Both degenerative phenomena were more frequent in stages III - VIII of seminiferous cycle, whereas at stages I and II only coiling of flagellum was observed. One week after ET, small amounts of EL were detected in stages IX - XII, suggesting spermiation failure. Signs of functional SC damage such as an accumulation of myelin-like inclusions in their cytoplasm were observed in the short but not medium-term. However, the apoptotic rates still remained five times higher and the number of elongated spermatids was three-fold lower. Our data indicate that exposure to a high dose of estrogen around puberty has stage-specific effects on the testis and causes massive degeneration of elongated spermatids. (c) 2007 Elsevier Ltd. All rights reserved. | |
| dc.language | eng | |
| dc.publisher | Churchill Livingstone | |
| dc.relation | Tissue & Cell | |
| dc.rights | info:eu-repo/semantics/closedAccess | |
| dc.subject | testis | |
| dc.subject | Estrogen | |
| dc.subject | Spermatogenesis | |
| dc.subject | Apoptosis | |
| dc.subject | Rat | |
| dc.title | Exposure of young rats to high estrogen doses leads to degeneration of elongated spermatids | |
| dc.type | Otro | |
