Minimal volume regulation after shrinkage of red blood cells from five species of reptiles

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorKristensen, Karina
dc.creatorBerenbrink, Michael
dc.creatorKoldkjaer, Pia
dc.creatorAbe, Augusto Shinya
dc.creatorWang, Tobias
dc.date2014-05-20T14:00:08Z
dc.date2016-10-25T17:07:49Z
dc.date2014-05-20T14:00:08Z
dc.date2016-10-25T17:07:49Z
dc.date2008-05-01
dc.date.accessioned2017-04-05T21:22:26Z
dc.date.available2017-04-05T21:22:26Z
dc.identifierComparative Biochemistry and Physiology A-molecular & Integrative Physiology. New York: Elsevier B.V., v. 150, n. 1, p. 46-51, 2008.
dc.identifier1095-6433
dc.identifierhttp://hdl.handle.net/11449/21268
dc.identifierhttp://acervodigital.unesp.br/handle/11449/21268
dc.identifier10.1016/j.cbpa.2008.03.002
dc.identifierWOS:000256499700008
dc.identifierhttp://dx.doi.org/10.1016/j.cbpa.2008.03.002
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/866857
dc.descriptionRed blood cells (RBCs) from most vertebrates restore volume upon hypertonic shrinkage and the mechanisms underlying this regulatory volume increase (RVI) have been studied extensively in these cells. Despite the phylogenetically interesting position of reptiles, very little is known about their red cell function. The present study demonstrates that oxygenated RBCs in all major groups of reptiles exhibit no or a very reduced RVI upon -25% calculated hyperosmotic shrinkage. Thus, RBCs from the snakes Crotalus durissus and Python regius, the turtle Trachemys scripta and the alligator Alligator mississippiensis showed no statistically significant RVI within 120 min after shrinkage, while the lizard Tupinambis merianae showed 22% volume recovery after 120 min. Amiloride (10(-4) M) and bumetanide (10(-5) M) had no effect on the RVI in T merianae, indicating no involvement of the Na(+)/H(+) exchanger (NHE) or the Na(+)/K(+)/2Cl(-) co-transporter (NKCC) or insentive transporters. Deoxygenation of RBCs from A. mississippiensis and T merianae did not significantly affect RVI upon shrinkage. Deoxygenation per se of red blood cells from T merianae elicited a slow volume increase, but the mechanism was not characterized. It seems, therefore, that the RVI response based on NHE activation was lost among the early sauropsids that gave rise to modern reptiles and birds, while it was retained in mammals. An RVI response has then reappeared in birds, but based on activation of the NKCC. Alternatively, the absence of the RVI response may represent the most ancient condition, and could have evolved several times within vertebrates. (C) 2008 Elsevier B.V. All rights reserved.
dc.languageeng
dc.publisherElsevier B.V.
dc.relationComparative Biochemistry and Physiology A-molecular & Integrative Physiology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectred blood cells
dc.subjectvolume regulation
dc.subjectregulatory volume increase
dc.subjectNa plus /H plus exchanger
dc.subjectNa+/K+/2Cl-co-transporter
dc.subjectreptile
dc.titleMinimal volume regulation after shrinkage of red blood cells from five species of reptiles
dc.typeOtro


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