dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorGomes, E.
dc.creatorSerzedello, A.
dc.creatorDa Silva, R.
dc.date2014-05-20T13:56:05Z
dc.date2016-10-25T17:05:33Z
dc.date2014-05-20T13:56:05Z
dc.date2016-10-25T17:05:33Z
dc.date1998-01-01
dc.date.accessioned2017-04-05T21:14:21Z
dc.date.available2017-04-05T21:14:21Z
dc.identifierMicrobios. Cambridge: Faculty Press, v. 96, n. 383, p. 39-50, 1998.
dc.identifier0026-2633
dc.identifierhttp://hdl.handle.net/11449/20060
dc.identifierhttp://acervodigital.unesp.br/handle/11449/20060
dc.identifierWOS:000078475500004
dc.identifierhttp://cat.inist.fr/?aModele=afficheN&cpsidt=1709661
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/865804
dc.descriptionA RNase of Aspergillus flavipes (IZ:1501) was purified from culture medium by chromatography on DEAE-cellulose and Sephadex G50 columns, after 96 h of cultivation. The molecular weight of the RNase was estimated to be 15 kD by gel filtration using Sephadex G100, and the optimum pH and temperature were 4.0 and 55 degrees C, respectively. Catalytic activity was inhibited by Hg2+, Ag+, Fe3+, Co2+ and Zn2+. The enzyme showed guanosine specificity producing only 3'-GMP from yeast RNA.
dc.languageeng
dc.publisherFaculty Press
dc.relationMicrobios
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectribonuclease
dc.subjectAspergillus
dc.subjectnucleotides
dc.titlePurification and physicochemical properties of a ribonuclease produced by Aspergillus flavipes (IZ : 1501)
dc.typeOtro


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