Investigation of cytotoxic, apoptosis-inducing, genotoxic and protective effects of the flavonoid rutin in HTC hepatic cells

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorMarcarini, Juliana Cristina
dc.creatorFerreira Tsuboy, Marcela Stefanini
dc.creatorLuiz, Rodrigo Cabral
dc.creatorRibeiro, Lucia Regina
dc.creatorHoffmann-Campo, Clara Beatriz
dc.creatorMantovani, Mario Segio
dc.date2014-05-20T13:55:45Z
dc.date2016-10-25T17:05:23Z
dc.date2014-05-20T13:55:45Z
dc.date2016-10-25T17:05:23Z
dc.date2011-07-01
dc.date.accessioned2017-04-05T21:13:51Z
dc.date.available2017-04-05T21:13:51Z
dc.identifierExperimental and Toxicologic Pathology. Jena: Elsevier Gmbh, Urban & Fischer Verlag, v. 63, n. 5, p. 459-465, 2011.
dc.identifier0940-2993
dc.identifierhttp://hdl.handle.net/11449/19968
dc.identifierhttp://acervodigital.unesp.br/handle/11449/19968
dc.identifier10.1016/j.etp.2010.03.005
dc.identifierWOS:000291706600007
dc.identifierhttp://dx.doi.org/10.1016/j.etp.2010.03.005
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/865734
dc.descriptionRutin is a flavonoid with antioxidant, vasodilatory, anti-inflammatory and immune-stimulating activities. To study the toxicity of rutin and its protective effect, this work investigated the cytotoxic, apoptosis-inducing, genotoxic and protective effects of rutin in HTC cells. In the MTT assay, the highest concentration tested (810 mu M) showed cytotoxicity after 72 h of treatment, where cell viability and cell proliferation was diminished. None of the concentrations of rutin tested induced apoptosis after 24 h treatment. The highest concentration of rutin after 24 h treatment induced DNA damage, shown in the comet assay, but did have a genotoxic effect in the micronucleus test. Rutin was tested against the pro-carcinogenic agent benzo(a)pyrene, at concentrations of 90, 270 and 810 mu M, and was found to reduce induced DNA damage significantly. This protective effect of rutin against a pro-carcinogen, suggests an important biological activity for this compound, which can contribute to human health through the diet. (C) 2010 Elsevier GmbH. All rights reserved.
dc.languageeng
dc.publisherElsevier Gmbh, Urban & Fischer Verlag
dc.relationExperimental and Toxicologic Pathology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectFlavonoid rutin
dc.subjectHTC
dc.subjectCytotoxicity
dc.subjectApoptosis
dc.subjectProtector effect
dc.titleInvestigation of cytotoxic, apoptosis-inducing, genotoxic and protective effects of the flavonoid rutin in HTC hepatic cells
dc.typeOtro


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