Histochemistry and Protein Profile of The Venom Glands of Workers of Neoponera villosa Ants (Hymenoptera: Ponerinae)

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorHenrique, Leopoldo Heitor
dc.creatorScopinho Furquim, Karim Christina
dc.creatorCamargo Mathias, Maria Izabel
dc.date2014-05-20T13:55:21Z
dc.date2016-10-25T17:05:07Z
dc.date2014-05-20T13:55:21Z
dc.date2016-10-25T17:05:07Z
dc.date2011-01-01
dc.date.accessioned2017-04-05T21:12:50Z
dc.date.available2017-04-05T21:12:50Z
dc.identifierSociobiology. Chico: California State Univ, v. 58, n. 3, p. 749-767, 2011.
dc.identifier0361-6525
dc.identifierhttp://hdl.handle.net/11449/19809
dc.identifierhttp://acervodigital.unesp.br/handle/11449/19809
dc.identifierWOS:000296689100017
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/865603
dc.descriptionCytochemical and histochemical tests applied to the venom glands of Neoponera villosa worker ants showed the presence of specific elements, such as lipids, polysaccharides (including glycogen), total protein, RNA, DNA and nucleolus organized regions (NORs). It was observed that protein is the most abundant element, since all portions of the gland reacted with strong positivity to the bromophenol blue test, especially the secretion, revealing the protein characteristics of the venom. Lipids were also present throughout the majority of the venom glands. The polysaccharides were more evident within the cells of the convoluted gland. The secretion, meanwhile, showed a weak positive reaction to this test. Despite the large number of protein elements in the secretion, was observed that the protein is not always in free form and is often associated with other elements, probably forming complexes. Tests to demonstrate RNA, DNA and NORs showed intense cell activity along the different portions of the gland and showed different stages of cell cycles. Tests for the dosage of protein in individuals collected in the summer (February-March) and winter (August-September) showed that there are differences in the total concentration of protein in the glands. Proteins with different molecular weights were detected in the final secreted product.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageeng
dc.publisherCalifornia State University
dc.relationSociobiology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectants
dc.subjectelectrophoresis
dc.subjectlipids
dc.subjectpolysaccharides
dc.subjectPonerinae
dc.subjectproteins
dc.subjectvenom glands
dc.titleHistochemistry and Protein Profile of The Venom Glands of Workers of Neoponera villosa Ants (Hymenoptera: Ponerinae)
dc.typeOtro


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