Characterization of two novel polyfunctional mastoparan peptides from the venom of the social wasp Polybia paulista

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorde Souza, Bibiana Monson
dc.creatorRodrigues da Silva, Alessandra Vaso
dc.creatorFerreira Resende, Virginia Maria
dc.creatorArcuri, Helen Andrade
dc.creatordos Santos Cabrera, Marcia Perez
dc.creatorRuggiero Neto, Joao
dc.creatorPalma, Mario Sergio
dc.date2014-05-20T13:55:05Z
dc.date2016-10-25T17:04:55Z
dc.date2014-05-20T13:55:05Z
dc.date2016-10-25T17:04:55Z
dc.date2009-08-01
dc.date.accessioned2017-04-05T21:12:05Z
dc.date.available2017-04-05T21:12:05Z
dc.identifierPeptides. New York: Elsevier B.V., v. 30, n. 8, p. 1387-1395, 2009.
dc.identifier0196-9781
dc.identifierhttp://hdl.handle.net/11449/19701
dc.identifierhttp://acervodigital.unesp.br/handle/11449/19701
dc.identifier10.1016/j.peptides.2009.05.008
dc.identifierWOS:000269038200001
dc.identifierhttp://dx.doi.org/10.1016/j.peptides.2009.05.008
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/865502
dc.descriptionHymenoptera venoms are complex mixtures of biochemically and pharmacologically active components such as biogenic amines, peptides and proteins. Polycationic peptides generally constitute the largest group of Hymenoptera venom toxins, and the mastoparans constitute the most abundant and important class of peptides in the venom of social wasps. These toxins are responsible for histamine release from mast cells, serotonin from platelets, and catecholamines and adenylic acids from adrenal chromafin cells. The present work reports the structural and functional characterization of two novel mastoparan peptides identified from the venom of the neotropical social wasp Polybia paulista. The mastoparans Polybia-MP-II and -III were purified, sequenced and synthesized on solid phase using Fmoc chemistry and the synthetic peptides used for structural and functional characterizations. Polybia-MP-II and -III are tetradecapeptides, amidated at their C-termini, and form amphipathic alpha-helical conformations under membrane-mimetic conditions. Both peptides were polyfunctional, causing pronounced cell lysis of rat mast cells and erythrocytes, in addition to having antimicrobial activity against both Gram-positive and Gram-negative bacteria. (C) 2009 Elsevier B.V. All rights reserved.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageeng
dc.publisherElsevier B.V.
dc.relationPeptides
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectWasp venom
dc.subjectToxins
dc.subjectMastoparan
dc.subjectPeptide-membrane interaction
dc.subjectCell lysis
dc.titleCharacterization of two novel polyfunctional mastoparan peptides from the venom of the social wasp Polybia paulista
dc.typeOtro


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