Application of the comet assay in erythrocytes of Oreochromis niloticus (Pisces): a methodological comparison

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorChristofoletti, Cintya A.
dc.creatorDavid, José Augusto O.
dc.creatorFontanetti, Carmem S.
dc.date2014-05-20T13:54:50Z
dc.date2014-05-20T13:54:50Z
dc.date2009-01-01
dc.date.accessioned2017-04-05T21:11:41Z
dc.date.available2017-04-05T21:11:41Z
dc.identifierGenetics and Molecular Biology. Sociedade Brasileira de Genética, v. 32, n. 1, p. 155-158, 2009.
dc.identifier1415-4757
dc.identifierhttp://hdl.handle.net/11449/19640
dc.identifier10.1590/S1415-47572009005000020
dc.identifierS1415-47572009000100024
dc.identifierWOS:000263377700024
dc.identifierS1415-47572009000100024.pdf
dc.identifierhttp://dx.doi.org/10.1590/S1415-47572009005000020
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/865448
dc.descriptionThe present study applied the comet assay to erythrocytes of Oreochromis niloticus with the aim of improving protocols to detect DNA damage in these cells, by using two distinct pHs (pH = 12.1 and pH > 13) and evaluating whether there is a correspondence between silver and ethidium bromide staining. Comets were visually examined and, the frequency of cells with and without damage was obtained, as well as the distribution of classes and scores. By using the Kruskal-Wallis test, our results revealed that pH 12.1 is more effective, although both pHs can be used. Our findings also suggest that silver staining can substitute ethidium bromide, an expensive and highly toxic stain that requires specific equipment for examination.
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageeng
dc.publisherSociedade Brasileira de Genética
dc.relationGenetics and Molecular Biology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectcomet assay
dc.subjectethidium bromide
dc.subjectsilver staining
dc.subjecttilapia
dc.titleApplication of the comet assay in erythrocytes of Oreochromis niloticus (Pisces): a methodological comparison
dc.typeOtro


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