Caenorhabditis elegans as a model to screen plant extracts and compounds as natural anthelmintics for veterinary use

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorKatiki, Luciana M.
dc.creatorFerreira, Jorge F. S.
dc.creatorZajac, Anne M.
dc.creatorMasler, Carol
dc.creatorLindsay, David S.
dc.creatorChagas, Ana Carolina S.
dc.creatorAmarante, Alessandro Francisco Talamini do
dc.date2014-05-20T13:53:13Z
dc.date2014-05-20T13:53:13Z
dc.date2011-12-15
dc.date.accessioned2017-04-05T21:08:21Z
dc.date.available2017-04-05T21:08:21Z
dc.identifierVeterinary Parasitology. Amsterdam: Elsevier B.V., v. 182, n. 2-4, p. 264-268, 2011.
dc.identifier0304-4017
dc.identifierhttp://hdl.handle.net/11449/18981
dc.identifier10.1016/j.vetpar.2011.05.020
dc.identifierWOS:000297524900017
dc.identifierWOS000297524900017.pdf
dc.identifierhttp://dx.doi.org/10.1016/j.vetpar.2011.05.020
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/864992
dc.descriptionThe most challenging obstacles to testing products for their anthelmintic activity are: (1) establishing a suitable nematode in vitro assay that can evaluate potential product use against a parasitic nematode of interest and (2) preparation of extracts that can be redissolved in solvents that are miscible in the test medium and are at concentrations well tolerated by the nematode system used for screening. The use of parasitic nematodes as a screening system is hindered by the difficulty of keeping them alive for long periods outside their host and by the need to keep infected animals as sources of eggs or adults when needed. This method uses the free-living soil nematode Caenorhabditis elegans as a system to screen products for their potential anthelmintic effect against small ruminant gastrointestinal nematodes, including Haemonchus contortus. This modified method uses only liquid axenic medium, instead of agar plates inoculated with Escherichia coil, and two selective sieves to obtain adult nematodes. During screening, the use of either balanced salt solution (M-9) or distilled water resulted in averages of 99.7 (+/- 0.73)% and 9636 (+/- 2.37)% motile adults, respectively. Adult worms tolerated DMSO, ethanol, methanol, and Tween 80 at 1% and 2%, while Labrasol (R) (a bioenhancer with low toxicity to mammals) and Tween 20 were toxic to C. elegans at 1% and were avoided as solvents. The high availability, ease of culture, and rapid proliferation of C elegans make it a useful screening system to test plant extracts and other phytochemical compounds to investigate their potential anthelmintic activity against parasitic nematodes. Published by Elsevier B.V.
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.languageeng
dc.publisherElsevier B.V.
dc.relationVeterinary Parasitology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectC. elegans
dc.subjectAnthelmintic plants
dc.subjectGastrointestinal nematodes
dc.subjectPlant extracts
dc.titleCaenorhabditis elegans as a model to screen plant extracts and compounds as natural anthelmintics for veterinary use
dc.typeOtro


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