Chromosomal painting and ZW sex chromosomes differentiation in Characidium (Characiformes, Crenuchidae)

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorMachado, Tatiana C.
dc.creatorPansonato-Alves, Jose C.
dc.creatorPucci, Marcela B.
dc.creatorNogaroto, Viviane
dc.creatorAlmeida, Mara C.
dc.creatorOliveira, Claudio
dc.creatorForesti, Fausto
dc.creatorBertollo, Luiz A. C.
dc.creatorMoreira-Filho, Orlando
dc.creatorArtoni, Roberto F.
dc.creatorVicari, Marcelo R.
dc.date2014-05-20T13:52:26Z
dc.date2014-05-20T13:52:26Z
dc.date2011-07-25
dc.date.accessioned2017-04-05T21:06:55Z
dc.date.available2017-04-05T21:06:55Z
dc.identifierBmc Genetics. London: Biomed Central Ltd., v. 12, p. 8, 2011.
dc.identifier1471-2156
dc.identifierhttp://hdl.handle.net/11449/18734
dc.identifier10.1186/1471-2156-12-65
dc.identifierWOS:000294203600001
dc.identifierWOS000294203600001.pdf
dc.identifierhttp://dx.doi.org/10.1186/1471-2156-12-65
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/864803
dc.descriptionBackground: The Characidium (a Neotropical fish group) have a conserved diploid number (2n = 50), but show remarkable differences among species and populations in relation to sex chromosome systems and location of nucleolus organizer regions (NOR). In this study, we isolated a W-specific probe for the Characidium and characterized six Characidium species/populations using cytogenetic procedures. We analyzed the origin and differentiation of sex and NOR-bearing chromosomes by chromosome painting in populations of Characidium to reveal their evolution, phylogeny, and biogeography.Results: A W-specific probe for efficient chromosome painting was isolated by microdissection and degenerate oligonucleotide primed-polymerase chain reaction (DOP-PCR) amplification of W chromosomes from C. gomesi. The W probe generated weak signals dispersed on the proto sex chromosomes in C. zebra, dispersed signals in both W and Z chromosomes in C. lauroi and, in C. gomesi populations revealed a proximal site on the long arms of the Z chromosome and the entire W chromosome. All populations showed small terminal W probe sites in some autosomes. The 18S rDNA revealed distinctive patterns for each analyzed species/population with regard to proto sex chromosome, sex chromosome pair, and autosome location.Conclusions: The results from dual-color fluorescence in situ hybridization (dual-color FISH) using W and 18S rDNA probes allowed us to infer the putative evolutionary pathways for the differentiation of sex chromosomes and NORs, from structural rearrangements in a sex proto-chromosome, followed by gene erosion and heterochromatin amplification, morphological differentiation of the sex chromosomal pair, and NOR transposition, giving rise to the distinctive patterns observed among species/populations of Characidium. Biogeographic isolation and differentiation of sex chromosomes seem to have played a major role in the speciation process in this group of fish.
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageeng
dc.publisherBiomed Central Ltd.
dc.relationBMC Genetics
dc.rightsinfo:eu-repo/semantics/openAccess
dc.titleChromosomal painting and ZW sex chromosomes differentiation in Characidium (Characiformes, Crenuchidae)
dc.typeOtro


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