dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorPinhal, Danillo
dc.creatorGadig, Otto Bismarck Fazzano
dc.creatorMartins, Cesar
dc.date2014-05-20T13:51:59Z
dc.date2016-10-25T17:03:13Z
dc.date2014-05-20T13:51:59Z
dc.date2016-10-25T17:03:13Z
dc.date2009-12-01
dc.date.accessioned2017-04-05T21:06:00Z
dc.date.available2017-04-05T21:06:00Z
dc.identifierConservation Genetics Resources. Dordrecht: Springer, v. 1, n. 1, p. 35-38, 2009.
dc.identifier1877-7252
dc.identifierhttp://hdl.handle.net/11449/18570
dc.identifierhttp://acervodigital.unesp.br/handle/11449/18570
dc.identifier10.1007/s12686-009-9008-9
dc.identifierWOS:000207922400009
dc.identifierhttp://dx.doi.org/10.1007/s12686-009-9008-9
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/864678
dc.descriptionA molecular approach based on nuclear 5S rDNA sequence variability was applied successfully to correctly identify samples from the two Rhizoprionodon species collected in the wild or sold in markets. The sequence of the non-transcribed spacer (NTS) of the 5S rDNA showed high interspecific variability and no intra-specific polymorphism, making it a useful marker for sharpnose shark identification. Polymorphisms in the NTS sequences of Rhizoprionodon sharks also created unique restriction patterns for each species after PCR-RFLP analysis. The 5S rDNA polymorphism represents a fast and non expensive tool to access species identification when rapid and unequivocal identification of shark products is needed, particularly for future management and other investigations.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageeng
dc.publisherSpringer
dc.relationConservation Genetics Resources
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectGenetic markers
dc.subjectNon-transcribed spacer
dc.subjectTrade monitoring
dc.subjectSharks
dc.subjectSpecies identification
dc.titleGenetic identification of the sharks Rhizoprionodon porosus and R. lalandii by PCR-RFLP and nucleotide sequence analyses of 5S rDNA
dc.typeOtro


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