Survey for Foot-and-mouth Disease in the Endangered Marsh Deer (Blastocerus dichotomus) from Marshlands of the Parana River Basin, Brazil

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorPessoa Araujo, Joao
dc.creatorNogueira, Marcia F.
dc.creatorDuarte, Jose M. B.
dc.date2014-05-20T13:50:58Z
dc.date2016-10-25T17:02:45Z
dc.date2014-05-20T13:50:58Z
dc.date2016-10-25T17:02:45Z
dc.date2010-07-01
dc.date.accessioned2017-04-05T21:04:16Z
dc.date.available2017-04-05T21:04:16Z
dc.identifierJournal of Wildlife Diseases. Lawrence: Wildlife Disease Assoc, Inc, v. 46, n. 3, p. 939-943, 2010.
dc.identifier0090-3558
dc.identifierhttp://hdl.handle.net/11449/18193
dc.identifierhttp://acervodigital.unesp.br/handle/11449/18193
dc.identifier10.7589/0090-3558-46.3.939
dc.identifierWOS:000280690400028
dc.identifierhttp://dx.doi.org/10.7589/0090-3558-46.3.939
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/864450
dc.descriptionHabitat fragmentation and diseases have resulted in a decline of the marsh deer (Blastocerus (dichotomus) throughout its South American range. Our objectives were to determine whether marsh deer intended for translocation from a region of the Rio Parana Basin had been infected previously by foot-and-mouth disease virus (FMDV) and whether they were carrying virus We captured marsh deer from June to October 1998 and collected blood from 108 animals and esophageal-pharyngeal fluid from 53 Serum was tested for antibodies against three FMDV serotypes (O, A, and C) by liquid-phase-blocking sandwich enzyme-linked immunosorbent assay (ELISA) Esophageal-pharyngeal fluid was tested for FMDV RNA by reverse transcription polymerase chain reaction (RT-PCR) and inoculation into three successive baby hamster kidney (BHK-21) cell subcultures, followed by RT-PCR of cultures We detected low log(10) titers (range 1 0-1 5) to FM DV subtype A(24) Cruzeiro in 19 of 108 sampled marsh deer, but failed to isolate FMDV or detect FMDV RNA in any samples we conclude that marsh deer from our study site were unlikely to carry FMDV, however, as a preventive measure, the 19 animals with titers for FMDV were not sent to FMDV-free Brazilian states
dc.languageeng
dc.publisherWildlife Disease Assoc, Inc
dc.relationJournal of Wildlife Diseases
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectBlastocerus dichotomus
dc.subjectELISA
dc.subjectFoot-and-mouth disease
dc.subjectMarsh deer
dc.subjectRio Parana Basin
dc.subjectRT-PCR
dc.subjectserologic survey
dc.titleSurvey for Foot-and-mouth Disease in the Endangered Marsh Deer (Blastocerus dichotomus) from Marshlands of the Parana River Basin, Brazil
dc.typeOtro


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