Th1/Th2 cytokines' expression and production by propolis-treated mice

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorOrsatti, Claudio Lera
dc.creatorMissima, Fabiane
dc.creatorPagliarone, Ana Carolina
dc.creatorSforcin, Jose Mauricio
dc.date2014-05-20T13:50:57Z
dc.date2016-10-25T17:02:45Z
dc.date2014-05-20T13:50:57Z
dc.date2016-10-25T17:02:45Z
dc.date2010-06-16
dc.date.accessioned2017-04-05T21:04:14Z
dc.date.available2017-04-05T21:04:14Z
dc.identifierJournal of Ethnopharmacology. Clare: Elsevier B.V., v. 129, n. 3, p. 314-318, 2010.
dc.identifier0378-8741
dc.identifierhttp://hdl.handle.net/11449/18183
dc.identifierhttp://acervodigital.unesp.br/handle/11449/18183
dc.identifier10.1016/j.jep.2010.03.030
dc.identifierWOS:000279439300005
dc.identifierhttp://dx.doi.org/10.1016/j.jep.2010.03.030
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/864445
dc.descriptionAim of the study: Propolis is a natural product extensively used in food and beverages to improve health and to prevent diseases, showing immunomodulatory properties. The goal of this work was to evaluate the effect of propolis administration over a short-term to mice on Th1 (IL-2 and IFN-gamma) and Th2 (IL-4 and IL-10) cytokines' expression and production.Materials and methods: Propolis was administered for 3 days to mice by gavage, spleens were removed and RNA was extracted to assess cytokines' expression by real-time PCR. Supernatants of spleen cell cultures were used for cytokines determination by ELISA.Results: Propolis administration to mice did not affect IL-2, IL-4 and IL-10 expression and production, while IFN-gamma production was inhibited in the splenocyte cultures stimulated or not by Con A.Conclusions: Since IFN-gamma is a pro-inflammatory cytokine, our data suggest that propolis administration over a short-term to mice may be associated with anti-inflammatory effects in vivo, and further assays could check propolis efficiency in inflammatory diseases. (C) 2010 Elsevier B.V. All rights reserved.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.languageeng
dc.publisherElsevier B.V.
dc.relationJournal of Ethnopharmacology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectPropolis
dc.subjectCytokine
dc.subjectImmunomodulation
dc.subjectReal-time PCR
dc.titleTh1/Th2 cytokines' expression and production by propolis-treated mice
dc.typeOtro


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