Interleukin-15 augments oxidative metabolism and fungicidal activity of human monocytes against Paracoccidioides brasiliensis

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorBannwart, Camila Ferreira
dc.creatorMartins, Rosana A Rodrigues
dc.creatorNakaira-Takahashi, Érika
dc.creatorDias-Melício, Luciane A.
dc.creatorSoares, Ângela MV Campos
dc.creatorPeraçoli, Maria Terezinha S.
dc.date2014-05-20T13:50:44Z
dc.date2014-05-20T13:50:44Z
dc.date2010-11-01
dc.date.accessioned2017-04-05T21:03:59Z
dc.date.available2017-04-05T21:03:59Z
dc.identifierMemórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 105, n. 7, p. 866-872, 2010.
dc.identifier0074-0276
dc.identifierhttp://hdl.handle.net/11449/18119
dc.identifier10.1590/S0074-02762010000700005
dc.identifierS0074-02762010000700005
dc.identifierWOS:000284778900005
dc.identifierS0074-02762010000700005.pdf
dc.identifierhttp://dx.doi.org/10.1590/S0074-02762010000700005
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/864410
dc.descriptionInterleukin (IL)-15 is a pleiotropic cytokine that regulates the proliferation and survival of many cell types. IL-15 is produced by monocytes and macrophages against infectious agents and plays a pivotal role in innate and adaptive immune responses. This study analyzed the effect of IL-15 on fungicidal activity, oxidative metabolism and cytokine production by human monocytes challenged in vitro with Paracoccidioides brasiliensis (Pb18), the agent of paracoccidioidomycosis. Peripheral blood monocytes were pre-incubated with IL-15 and then challenged with Pb18. Fungicidal activity was assessed by viable fungi recovery from cultures after plating on brain-heart infusion-agar. Superoxide anion (O2-), hydrogen peroxide (H2O2), tumour necrosis factor-alpha (TNF-α), IL-6, IL-15 and IL-10 production by monocytes were also determined. IL-15 enhanced fungicidal activity against Pb18 in a dose-dependent pattern. This effect was abrogated by addition of anti-IL-15 monoclonal antibody. A significant stimulatory effect of IL-15 on O2- and H2O2 release suggests that fungicidal activity was dependent on the activation of oxidative metabolism. Pre-treatment of monocytes with IL-15 induced significantly higher levels of TNF-α, IL-10 and IL-15 production by cells challenged with the fungus. These results suggest a modulatory effect of IL-15 on pro and anti-inflammatory cytokine production, oxidative metabolism and fungicidal activity of monocytes during Pb18 infection.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageeng
dc.publisherInstituto Oswaldo Cruz, Ministério da Saúde
dc.relationMemórias do Instituto Oswaldo Cruz
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectIL-15
dc.subjectParacoccidioides brasiliensis
dc.subjectmonocytes
dc.subjecthydrogen peroxide
dc.subjectfungicidal activity
dc.subjectcytokines
dc.titleInterleukin-15 augments oxidative metabolism and fungicidal activity of human monocytes against Paracoccidioides brasiliensis
dc.typeOtro


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