Bioinformatical and in vitro approaches to essential oil-induced matrix metalloproteinase inhibition

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorZeidan-Chulia, Fares
dc.creatorRybarczyk-Filho, Jose L.
dc.creatorGursoy, Mervi
dc.creatorKononen, Eija
dc.creatorUitto, Veli-Jukka
dc.creatorGursoy, Orhan V.
dc.creatorCakmakci, Lutfu
dc.creatorMoreira, Jose C. F.
dc.creatorGursoy, Ulvi K.
dc.date2014-05-20T13:49:32Z
dc.date2016-10-25T17:01:59Z
dc.date2014-05-20T13:49:32Z
dc.date2016-10-25T17:01:59Z
dc.date2012-06-01
dc.date.accessioned2017-04-05T21:01:30Z
dc.date.available2017-04-05T21:01:30Z
dc.identifierPharmaceutical Biology. London: Informa Healthcare, v. 50, n. 6, p. 675-686, 2012.
dc.identifier1388-0209
dc.identifierhttp://hdl.handle.net/11449/17660
dc.identifierhttp://acervodigital.unesp.br/handle/11449/17660
dc.identifier10.3109/13880209.2012.677847
dc.identifierWOS:000304108500001
dc.identifierhttp://dx.doi.org/10.3109/13880209.2012.677847
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/864089
dc.descriptionContext: Essential oils carry diverse antimicrobial and anti-enzymatic properties.Objective: Matrix metalloproteinase (MMP) inhibition characteristics of Salvia fruticosa Miller (Labiatae), Myrtus communis Linnaeus (Myrtaceae), Juniperus communis Linnaeus (Cupressaceae), and Lavandula stoechas Linnaeus (Labiatae) essential oils were evaluated.Materials and methods: Chemical compositions of the essential oils were analyzed by gas chromatography-mass spectrometry (GC-MS). Bioinformatical database analysis was performed by STRING 9.0 and STITCH 2.0 databases, and ViaComplex software. Antibacterial activity of essential oils against periodontopathogens was tested by the disc diffusion assay and the agar dilution method. Cellular proliferation and cytotoxicity were determined by commercial kits. MMP-2 and MMP-9 activities were measured by zymography.Results: Bioinformatical database analyses, under a score of 0.4 (medium) and a prior correction of 0.0, gave rise to a model of protein (MMPs and tissue inhibitors of metalloproteinases) vs. chemical (essential oil components) interaction network; where MMPs and essential oil components interconnected through interaction with hydroxyl radicals, molecular oxygen, and hydrogen peroxide. Components from L. stoechas potentially displayed a higher grade of interaction with MMP-2 and -9. Although antibacterial and growth inhibitory effects of essential oils on the tested periodontopathogens were limited, all of them inhibited MMP-2 in vitro at concentrations of 1 and 5 mu L/mL. Moreover, same concentrations of M. communis and L. stoechas also inhibited MMP-9. MMP-inhibiting concentrations of essential oils were not cytotoxic against keratinocytes.Discussion and conclusion: We propose essential oils of being useful therapeutic agents as MMP inhibitors through a mechanism possibly based on their antioxidant potential.
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.languageeng
dc.publisherInforma Healthcare
dc.relationPharmaceutical Biology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectSalvia fruticosa M.
dc.subjectMyrtus communis L.
dc.subjectJuniperus communis L.
dc.subjectLavandula stoechas L.
dc.subjectMMP-2
dc.subjectMMP-9
dc.subjectsystems biology
dc.subjectperiodontitis
dc.titleBioinformatical and in vitro approaches to essential oil-induced matrix metalloproteinase inhibition
dc.typeOtro


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