Rapid method for simultaneous determination of CIS-9, TRANS-11 and TRANS-10, CIS-12 conjugated linoleic acid isomers in milk by GC-FID.

dc.contributorBRENDA LEE SIMAS PORTO, Universidade Federal de Juiz de Fora
dc.contributorISAURA DANIELE LEITE FARIA, Universidade Federal de Juiz de Fora
dc.contributorJÉSSICA CORDEIRO QUEIROZ DE SOUZA, Universidade Federal de Juiz de Fora
dc.contributorOLÍVIA BRITO DE OLIVEIRA MOREIRA, Universidade Federal de Juiz de Fora
dc.contributorMARCO ANTONIO SUNDFELD DA GAMA, CPPSE
dc.contributorMARCONE AUGUSTO LEAL DE OLIVEIRA, Universidade Estadual de Campinas.
dc.creatorPORTO, B. L. S.
dc.creatorFARIA, I. D. L.
dc.creatorSOUZA, J. C. Q. DE
dc.creatorMOREIRA, O. B. DE O.
dc.creatorGAMA, M. A. S. da
dc.creatorOLIVEIRA, M. A. L. DE
dc.date2023-08-28T16:23:41Z
dc.date2023-08-28T16:23:41Z
dc.date2023-08-28
dc.date2023
dc.date.accessioned2023-09-05T03:07:53Z
dc.date.available2023-09-05T03:07:53Z
dc.identifierQuímica Nova, v. 7, p. 743-746, 2023.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1156165
dc.identifier10.21577/0100-4042.20230026
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8638586
dc.descriptionRuminant milk is the lead source of conjugated linoleic acid (CLA) in the human diet, with cis-9, trans-11 CLA being the major among all. Small amounts of trans-10, cis-12 CLA are also found in synthetic supplements. Since both isomers are biologically active with potential health benefits, there is great interest in quantifying them for quality control routines. An alternative method for the analysis of the aforementioned CLAs by fast gas chromatography (GC) is discussed in the present study. The fatty acid methyl ester mixture obtained by alkaline catalysis was injected into a GC equipped with a flame ionization detector (FID) and fitted with an ionic liquid SLB-IL111 chromatographic column (15 m × 0.10 mm × 0.08 µm). Separation was achieved in less than 5 min using a 168 °C isotherm run. Both CLA isomers were quantified by using of single point standard addition statistical approach. Results were contested to those obtained using the recommended 100 m long CP-Sil88 capillary column and none evidence of significant differences was found within 95% confidence interval. Therefore, the proposed method could be valuable to focused regulatory routines of large numbers of samples with greater analytical frequency.
dc.languageIngles
dc.languageen
dc.rightsopenAccess
dc.subjectFast GC
dc.subjectCLA
dc.subjectSLB IL111
dc.subjectMilk
dc.subjectFatty acids
dc.titleRapid method for simultaneous determination of CIS-9, TRANS-11 and TRANS-10, CIS-12 conjugated linoleic acid isomers in milk by GC-FID.
dc.typeArtigo de periódico


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