| dc.contributor | Oklahoma University; Eliane Cristina Locali-Fabris, APTA; JULIANA DE FREITAS ASTUA, CNPMF. | |
| dc.creator | OCHOA-CORONA, F. M. | |
| dc.creator | LOCALI-FABRIS, E. C. | |
| dc.creator | FREITAS-ASTÚA, J. | |
| dc.date | 2023-08-02T18:23:45Z | |
| dc.date | 2023-08-02T18:23:45Z | |
| dc.date | 2010-05-03 | |
| dc.date | 2009 | |
| dc.date.accessioned | 2023-09-05T02:59:32Z | |
| dc.date.available | 2023-09-05T02:59:32Z | |
| dc.identifier | Phytopathology, St. Paul, v. 99, n. 6, S28, 2009. Suplement | |
| dc.identifier | http://www.alice.cnptia.embrapa.br/alice/handle/doc/748515 | |
| dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/8638141 | |
| dc.description | Citrus leprosis virus C (CiLV-C), which is transmitted both mechanically and by the mite Brevipalpus phoenicis (Geijskes) (Acari: Tenuipalpidae), causes significant disease damage in South and Central America. Citrus leprosis disease was first recorded in Florida in 1925, but is believed eradicated through improved mite control procedures. CiLV-C is a threat to citrus producing nations where it is not present, such as New Zealand and the USA, and a sensitive detection method is required for screening and biosecurity of suspect quarantine material. CiLV-C is mechanically transmitted, posses a bipartite RNA genome and was believed to be a rhabdovirus. After being sequenced, CiLV-C was proposed as the type member of a new genus, Cilevirus, related to several (+) ssRNA viruses. Of two known morphological types of CiLV particles, the cytoplasmic type (CiLV-C) is prevalent than the nuclear type (CiLV-N) in Brazil and elsewhere. A pair of diagnostic primers, amplifying a segment of 278 bp located at the RNA-2 p15 gene of CiLV-C was designed using the Web software pathway Primer3-mFOLD-BLASTn. A thermodynamically robust RT-PCR that performs well in a range of melting temperatures and specifically optimized for CiLV-C was developed, and is a feasible tool to be used in quarantine. | |
| dc.description | Abstracts of presentations, Annual Meeting of the APS 2009. p. S28. | |
| dc.language | Ingles | |
| dc.language | en | |
| dc.rights | openAccess | |
| dc.subject | Brevipalpus Phoenicis | |
| dc.subject | Fruta Cítrica | |
| dc.subject | Citrus | |
| dc.title | An RT-PCR procedure for detection and surveillance of Citrus leprosis virus C (CiLV-C) in post-entry quarantine stocks of citrus. | |
| dc.type | Resumo em anais e proceedings | |
