Jaw and Long Bone Marrows Have a Different Osteoclastogenic Potential

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorde Souza Faloni, Ana Paula
dc.creatorSchoenmaker, Ton
dc.creatorAzari, Azin
dc.creatorKatchburian, Eduardo
dc.creatorCerri, Paulo Sérgio
dc.creatorde Vries, Teun J.
dc.creatorEverts, Vincent
dc.date2014-05-20T13:46:52Z
dc.date2014-05-20T13:46:52Z
dc.date2011-01-01
dc.date.accessioned2017-04-05T20:56:02Z
dc.date.available2017-04-05T20:56:02Z
dc.identifierCalcified Tissue International. New York: Springer, v. 88, n. 1, p. 63-74, 2011.
dc.identifier0171-967X
dc.identifierhttp://hdl.handle.net/11449/16617
dc.identifier10.1007/s00223-010-9418-4
dc.identifierWOS:000286201200009
dc.identifierWOS000286201200009.pdf
dc.identifierhttp://dx.doi.org/10.1007/s00223-010-9418-4
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/863363
dc.descriptionOsteoclasts, the multinucleated bone-resorbing cells, arise through fusion of precursors from the myeloid lineage. However, not all osteoclasts are alike; osteoclasts at different bone sites appear to differ in numerous respects. We investigated whether bone marrow cells obtained from jaw and long bone differed in their osteoclastogenic potential. Bone marrow cells from murine mandible and tibiae were isolated and cultured for 4 and 6 days on plastic or 6 and 10 days on dentin. Osteoclastogenesis was assessed by counting the number of TRAP(+) multinucleated cells. Bone marrow cell composition was analyzed by FACS. The expression of osteoclast- and osteoclastogenesis-related genes was studied by qPCR. TRAP activity and resorptive activity of osteoclasts were measured by absorbance and morphometric analyses, respectively. At day 4 more osteoclasts were formed in long bone cultures than in jaw cultures. At day 6 the difference in number was no longer observed. The jaw cultures, however, contained more large osteoclasts on plastic and on dentin. Long bone marrow contained more osteoclast precursors, in particular the myeloid blasts, and qPCR revealed that the RANKL:OPG ratio was higher in long bone cultures. TRAP expression was higher for the long bone cultures on dentin. Although jaw osteoclasts were larger than long bone osteoclasts, no differences were found between their resorptive activities. In conclusion, bone marrow cells from different skeletal locations (jaw and long bone) have different dynamics of osteoclastogenesis. We propose that this is primarily due to differences in the cellular composition of the bone site-specific marrow.
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageeng
dc.publisherSpringer
dc.relationCalcified Tissue International
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectJaw
dc.subjectLong bone
dc.subjectOsteoclastogenesis
dc.subjectOsteoclast precursor
dc.subjectHeterogeneity
dc.subjectMarrow
dc.titleJaw and Long Bone Marrows Have a Different Osteoclastogenic Potential
dc.typeOtro


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