Characterization of Staphylococcus spp. strains in milk from buffaloes with mastitis in Brazil: the need to identify to species level to avoid misidentification.

dc.contributorUFRRJ; MARIA APARECIDA V PAIVA E BRITO, CNPGL; UFRRJ; UFRRJ; UFRRJ.
dc.creatorCOIMBRA E SOUZA, V.
dc.creatorBRITO, M. A. V. P. e
dc.creatorCHAMON, R. C.
dc.creatorLAPORT, M. S.
dc.creatorGIAMBIAGI DE MARVAL, M.
dc.date2022-08-23T10:20:10Z
dc.date2022-08-23T10:20:10Z
dc.date2017-12-22
dc.date2017
dc.date.accessioned2023-09-05T01:39:36Z
dc.date.available2023-09-05T01:39:36Z
dc.identifierArquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 69, n. 6, p. 1669-1675, 2017.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1083432
dc.identifierhttps://doi.org/10.1590/1678-4162-9351
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8631667
dc.descriptionABSTRACT - Mastitis is an inflammation of the mammary gland that affects dairy cattle worldwide causing economic losses. Coagulase-negative staphylococci (CNS) are the predominant cause of this type of infection. We have recently showed that coagulase-positive staphylococci could be misidentified. So, the aim of this study was to characterize the Staphylococcus spp. strains initially classified as coagulase-negative Staphylococci, isolated from buffalo with subclinical mastitis. Milk of buffaloes with mastitis in herds was collected and 9 strains were identified as CNS by phenotypic tests. Molecular methodologies latter identified the strains as coagulase-negative Staphylococcus chromogenes (5), coagulase-positive Staphylococcus hyicus (2) and coagulase-positive Staphylococcus aureus (2). Our results strongly support the need to identify the isolates to a species level in order to avoid misidentification and to be aware of the classification using the coagulase test alone. RESUMO - A mastite é uma inflamação da glândula mamária que afeta o gado leiteiro em todo o mundo, causando perdas econômicas. Staphylococcus coagulase-negativa (SCN) são a causa predominante desse tipo de infecção. Mostrou-se recentemente que Staphylococcus coagulase-positiva podem ser identificados erroneamente. Assim, o objetivo deste estudo foi caracterizar cepas de Staphylococcus spp. inicialmente classificados como Staphylococcus coagulase-negativa, isolados de búfalas com mastite subclínica. O leite de búfalas com mastite foi coletado, e nove cepas foram identificadas como SCN por testes fenotípicos. Metodologias moleculares identificaram as cepas como Staphylococcus chromogenes coagulase-negativa (5) Staphylococcus hyicus coagulase-positiva (2) e Staphylococcus aureus coagulase-positiva (2). Os resultados reforçam a necessidade de identificar as cepas em termos de espécie, a fim de se evitarem erros de identificação e estar atento à classificação utilizando o teste de coagulase sozinho.
dc.languageIngles
dc.languageen
dc.rightsopenAccess
dc.subjectBuffalo
dc.subjectCoagulase test
dc.subjectStaphylococcus
dc.subjectmastitis
dc.subjectmilk
dc.titleCharacterization of Staphylococcus spp. strains in milk from buffaloes with mastitis in Brazil: the need to identify to species level to avoid misidentification.
dc.typeArtigo de periódico


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