AT(1) receptor blockade in the lateral parabrachial nucleus reduces the effects of muscimol on sodium intake

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorDa Silva, Camila Zambone C.
dc.creatorMenani, José Vanderlei
dc.creatorCallera, Joao Carlos
dc.date2014-05-20T13:46:15Z
dc.date2014-05-20T13:46:15Z
dc.date2011-07-27
dc.date.accessioned2017-04-05T20:54:34Z
dc.date.available2017-04-05T20:54:34Z
dc.identifierBrain Research. Amsterdam: Elsevier B.V., v. 1403, p. 28-36, 2011.
dc.identifier0006-8993
dc.identifierhttp://hdl.handle.net/11449/16342
dc.identifier10.1016/j.brainres.2011.06.004
dc.identifierWOS:000293156800004
dc.identifierWOS000293156800004.pdf
dc.identifierhttp://dx.doi.org/10.1016/j.brainres.2011.06.004
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/863162
dc.descriptionThe blockade of the lateral parabrachial nucleus (LPBN) with GABA(A) receptor agonist muscimol induces robust hypertonic NaCl and water intake by rats. In the present study we investigated the effects of previous injections of losartan (AT(1) angiotensin receptor antagonist) into the LPBN on 0.3 M NaCl and water intake induced by muscimol injected bilaterally in the same area in fluid replete rats and in rats treated with the diuretic furosemide combined with a low dose of the angiotensin-converting enzyme inhibitor captopril injected subcutaneously. Male Wistar rats with stainless steel cannulas implanted bilaterally into the LPBN were used. Bilateral injections of muscimol (0.5 nmol/0.2 mu l, n=8) into the LPBN in fluid replete rats induced 0.3 M NaCl intake (23.4 +/- 4.1 vs. saline: 0.4 +/- 0.4 ml/3 h) and water intake (9.3 +/- 1.9 vs. saline: 0.7 +/- 0.4 ml/3 h) and pre-treatment of the LPBN with losartan (50 mu g/0.2 mu l) reduced 0.3 M NaCl intake (3.3 +/- 2.5 ml/3 h) and water intake (4.0 +/- 2.9 ml/3 h) induced by muscimol. In rats treated with furosemide + captopril, pretreatment with losartan into the LPBN attenuated the increase of 0.3 M NaCl intake produced by muscimol (12.8 +/- 5.3, vs. saline + muscimol: 36.7 +/- 6.7 ml/3 h) without changing water intake. Therefore, the results suggest that deactivation of LPBN inhibitory mechanisms by muscimol injections into the LPBN is facilitated by endogenous angiotensin II acting on AT(1) receptors in the LPBN, which drives rats to ingest large amounts of hypertonic NaCl. (C) 2011 Elsevier B.V. All rights reserved.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageeng
dc.publisherElsevier B.V.
dc.relationBrain Research
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectGABA receptor
dc.subjectAngiotensin II
dc.subjectLosartan
dc.subjectSodium appetite
dc.subjectThirst
dc.subjectLateral parabrachial nucleus
dc.titleAT(1) receptor blockade in the lateral parabrachial nucleus reduces the effects of muscimol on sodium intake
dc.typeOtro


Este ítem pertenece a la siguiente institución