dc.contributorJosé Vicente Die, IFAPA; Belén Román, IFAPA; Nadal Moyano Salvador, IFAPA; MIGUEL ANGEL DITA RODRIGUEZ, CNPMF; Clara Isabel González-Verdejo, IFAPA.
dc.creatorDIE, J. V.
dc.creatorROMAN, B.
dc.creatorSALVADOR, N.
dc.creatorRODRIGUEZ, M. A. D.
dc.creatorGONZÁLEZ-VERDEJO, C. I.
dc.date2022-08-22T17:22:53Z
dc.date2022-08-22T17:22:53Z
dc.date2009-07-06
dc.date2009
dc.date.accessioned2023-09-05T01:39:13Z
dc.date.available2023-09-05T01:39:13Z
dc.identifierCrop and Pasture Science, Victoria, v. 60, n. 5, p. 490-498, mai. 2009.
dc.identifier1836-0947
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/655752
dc.identifierDOI: 10.1071/CP08274
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8631626
dc.descriptionThe root holoparasitic angiosperm Orobanche crenata is a severe constraint to the cultivation of legumes. Breeding for resistance is a difficult task. Understanding the mechanisms underlying host resistance is a fundamental issue for the genetic improvement of legumes. In this work, the temporal expression patterns of 8 defence-genes known to be involved in different metabolic pathways activated during several plant-pathogen interactions were investigated in Pisum sativum. Molecular analyses were carried out using quantitative real-time polymerase chain reaction during the initial stages of the parasitisation process in susceptible (Messire) and incompletely resistant (Ps624) pea genotypes. Transcriptional changes in response to O. crenata revealed induction of genes putatively encoding pathogenesis-related proteins, peroxidase activity, and dehydration stress-responsive signalling. This, combined with high constitutive gene expression mediating the phenylpropanoid pathway were observed as part of the defence mechanisms triggered in Ps624 to restrict the growth of the parasite.
dc.languageIngles
dc.languageen
dc.rightsopenAccess
dc.subjectPlant defence
dc.subjectReal-time PCR
dc.subjectMelhoramento Genético Vegetal
dc.subjectPatógeno
dc.subjectparasitic plants
dc.titleExpression analysis of Pisum sativum putative defence genes during Orobanche crenata infection.
dc.typeArtigo de periódico


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