Genome-wide association study for Haemonchus contortus resistance in Morada Nova sheep.

dc.contributorSIMONE CRISTINA MEO NICIURA, CPPSE; MAGDA VIEIRA BENAVIDES, CPPSUL; CINTIA HIROMI OKINO, CPPSE; ADRIANA MERCIA GUARATINI IBELLI, CNPSA; ALESSANDRO PELEGRINE MINHO, CPPSE; SERGIO NOVITA ESTEVES, CPPSE; ANA CAROLINA DE SOUZA CHAGAS, CPPSE.
dc.creatorNICIURA, S. C. M.
dc.creatorBENAVIDES, M. V.
dc.creatorOKINO, C. H.
dc.creatorIBELLI, A. M. G.
dc.creatorMINHO, A. P.
dc.creatorESTEVES, S. N.
dc.creatorCHAGAS, A. C. de S.
dc.date2022-08-22T12:20:18Z
dc.date2022-08-22T12:20:18Z
dc.date2022-08-22
dc.date2022
dc.date.accessioned2023-09-05T01:38:46Z
dc.date.available2023-09-05T01:38:46Z
dc.identifierPathogens, v. 11, n. 8, aug. 2022, 939.
dc.identifierhttp://www.alice.cnptia.embrapa.br/alice/handle/doc/1145606
dc.identifierhttps://doi.org/10.3390/pathogens11080939
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8631588
dc.descriptionAmong the gastrointestinal nematodes affecting sheep, Haemonchus contortus is the most prevalent and virulent, resulting in health problems and production losses. Therefore, selecting sheep resistant to H. contortus is a suitable and sustainable strategy for controlling endoparasites in flocks. Here, 287 lambs of the native Brazilian Morada Nova hair sheep breed were subjected to two consecutive artificial infections with H. contortus and assessed for fecal egg count (FEC), packed cell volume (PCV), and live weight (LW). Forty-four animals ranked as having extreme resistance phenotypes were genotyped using the Illumina OvineSNP50v3 chip. A case?control genome-wide association study (GWAS) detected 37 significant (p < 0.001) markers in 12 ovine chromosomes in regions harboring quantitative trait loci (QTL) for FEC, Trichostrongylus spp. adults and larvae, weight, and fat; and candidate genes for immune responses, mucins, hematological parameters, homeostasis, and growth. Four single-nucleotide polymorphisms (SNP; OAR1_rs427671974, AR2_rs419988472, OAR5_rs424070217, and OAR17_rs401006318) genotyped by qPCR followed by high-resolution melting (HRM) were associated with FEC and LW. Therefore, molecular markers detected by GWAS for H. contortus resistance in Morada Nova sheep may support animal selection programs aimed at controlling gastrointestinal nematode infections in flocks. Furthermore, genotyping of candidate genes using HRM qPCR may provide a rapid and efficient tool for animal identification.
dc.format12 p.
dc.languageIngles
dc.languageen
dc.rightsopenAccess
dc.subjectGastrointestinal nematode control
dc.subjectGWAS
dc.subjectMolecular markers
dc.subjectOvine
dc.subjectParasite resistance
dc.subjectGenotyping
dc.titleGenome-wide association study for Haemonchus contortus resistance in Morada Nova sheep.
dc.typeArtigo de periódico


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