DETECTION of ANTIBODIES in SERA FROM CHAGAS-DISEASE PATIENTS USING A TRYPANOSOMA-CRUZI IMMUNODOMINANT RECOMBINANT ANTIGEN

dc.contributorUniversidade Federal de São Paulo (UNIFESP)
dc.contributorBIOMERIEUX
dc.contributorBIOLAB DIAGNOST
dc.contributorUniversidade Federal de Goiás (UFG)
dc.contributorUniversidade de São Paulo (USP)
dc.creatorParanhosbacalla, G. S.
dc.creatorSantos, MRM
dc.creatorCotrim, P.
dc.creatorRassi, A.
dc.creatorJolivet, M.
dc.creatorCamargo, M. E.
dc.creatorDasilveira, J. F.
dc.date.accessioned2016-01-24T11:40:17Z
dc.date.accessioned2023-09-04T19:20:04Z
dc.date.available2016-01-24T11:40:17Z
dc.date.available2023-09-04T19:20:04Z
dc.date.created2016-01-24T11:40:17Z
dc.date.issued1994-03-01
dc.identifierParasite Immunology. Oxford: Blackwell Science Ltd, v. 16, n. 3, p. 165-169, 1994.
dc.identifier0141-9838
dc.identifierhttp://repositorio.unifesp.br/handle/11600/25389
dc.identifier10.1111/j.1365-3024.1994.tb00336.x
dc.identifierWOS:A1994NC54300006
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8625319
dc.description.abstractA Trypanosoma cruzi DNA fragment encoding an immunodominant repetitive antigen (H49) was subcloned into a protein purification and expressed system. Purified H49 peptide reacted specifically in an enzyme-linked immunosorbent assay (ELISA) with sera from T. cruzi-infected patients, but not with sera from patients with other parasitic diseases such as leishmaniasis and T. rangeli-infection. the H49 recombinant ELISA was able to detect specific antibodies in 84% of chronic chagasic serum samples tested. One of the major advantage of the recombinant ELISA for serodiagnosis of chronic Chagas' disease resides in its high specificity (100%). Our data suggest that recombinant peptides could provide a practical basis for specific diagnosis tests for Chagas' disease.
dc.languageeng
dc.publisherBlackwell Science Ltd
dc.relationParasite Immunology
dc.rightsAcesso restrito
dc.subjectCHAGAS DISEASE
dc.subjectRECOMBINANT ANTIGEN
dc.subjectIMMUNODOMINANT REPETITIVE ANTIGEN
dc.subjectELISA
dc.subjectSERODIAGNOSIS
dc.titleDETECTION of ANTIBODIES in SERA FROM CHAGAS-DISEASE PATIENTS USING A TRYPANOSOMA-CRUZI IMMUNODOMINANT RECOMBINANT ANTIGEN
dc.typeArtigo


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