Granulocyte-macrophage colony-stimulating factor gene based therapy for acute limb ischemia in a mouse model
dc.contributor | Universidade Federal de São Paulo (UNIFESP) | |
dc.contributor | Universidade Federal do Rio de Janeiro (UFRJ) | |
dc.contributor | Univ Fed Rio Grande do Sul | |
dc.contributor | Excell Biomed Serv | |
dc.creator | Sacramento, Chester Bittencourt [UNIFESP] | |
dc.creator | Cantagalli, Vanessa Dionisio [UNIFESP] | |
dc.creator | Grings, Mariana [UNIFESP] | |
dc.creator | Carvalho, Leonardo Pinto [UNIFESP] | |
dc.creator | Costa Baptista-Silva, Jose Carlos [UNIFESP] | |
dc.creator | Beutel, Abram [UNIFESP] | |
dc.creator | Bergamaschi, Cassia Toledo [UNIFESP] | |
dc.creator | Campos, Ruy Ribeiro [UNIFESP] | |
dc.creator | Moraes, Jane Zveiter de [UNIFESP] | |
dc.creator | Takiya, Christina Maeda | |
dc.creator | Samoto, Vivian Yochiko | |
dc.creator | Borojevic, Radovan | |
dc.creator | Silva, Flavia Helena da [UNIFESP] | |
dc.creator | Nardi, Nance Beyer | |
dc.creator | Dohmann, Hans Fernando | |
dc.creator | Silva Junior, Hamilton | |
dc.creator | Valero, Valderez Bastos [UNIFESP] | |
dc.creator | Han, Sang Won [UNIFESP] | |
dc.date.accessioned | 2016-01-24T13:52:23Z | |
dc.date.accessioned | 2023-09-04T19:17:31Z | |
dc.date.available | 2016-01-24T13:52:23Z | |
dc.date.available | 2023-09-04T19:17:31Z | |
dc.date.created | 2016-01-24T13:52:23Z | |
dc.date.issued | 2009-04-01 | |
dc.identifier | Journal of Gene Medicine. Chichester: John Wiley & Sons Ltd, v. 11, n. 4, p. 345-353, 2009. | |
dc.identifier | 1099-498X | |
dc.identifier | http://repositorio.unifesp.br/handle/11600/31405 | |
dc.identifier | 10.1002/jgm.1298 | |
dc.identifier | WOS:000265249800007 | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/8624896 | |
dc.description.abstract | Background Granulocyte-colony-stimulating factor (GM-CSF) is a pleiotropic factor for hematopoiesis that stimulates myeloblasts, monoblasts and mobilization of bone marrow stem cells. Therefore, the GM-CSF gene is a potential candidate for vessel formation and tissue remodeling in the treatment of ischemic diseases.Methods A new mouse limb ischemia was established by surgery and gene transfer was performed by injection of 100 mu g of a plasmid carrying GM-CSF. Muscle force and weight, histology, capillary density, circulating stem cells and monocytes were determined after 3-4 weeks.Results More than 60% of nontreated ischemic animals showed gangrene below the heel after 4 weeks, whereas the GM-CSF gene-treated animals showed only darkening of nails or toes. These animals demonstrated a full recovery of the affected muscles in terms of weight, force and muscle fiber structure, but the muscles of nontreated ischemic animals lost approximately 50% weight, 86% force and their regular structure. When the GM-CSF gene was injected into the contralateral limb, only partial loss was observed, demonstrating a distant effect of GM-CSF. the capillary density in the GM-CSF-treated group was 52% higher in relation to the nontreated group. Blood analysis by flow cytometry showed that the GMCSF-treated group had 10-20% higher levels of circulating monocytes and Sca-1(+).Conclusions We conclude that the direct administration of GM-CSF gene in limb ischemia had a strong therapeutic effect because it promoted the recovery of muscle mass, force and structure by mobilizing therapeutic cells and augmenting the number of vessels. Copyright (C) 2009 John Wiley & Sons, Ltd. | |
dc.language | eng | |
dc.publisher | Wiley-Blackwell | |
dc.relation | Journal of Gene Medicine | |
dc.rights | http://olabout.wiley.com/WileyCDA/Section/id-406071.html | |
dc.rights | Acesso restrito | |
dc.subject | Angiogenesis | |
dc.subject | Arteriogenesis | |
dc.subject | Gene therapy | |
dc.subject | GM-CSF | |
dc.subject | Ischemia | |
dc.subject | Vasculogenesis | |
dc.title | Granulocyte-macrophage colony-stimulating factor gene based therapy for acute limb ischemia in a mouse model | |
dc.type | Artigo |