Natriorexigenic effect of baclofen is reduced by AT(1) receptor blockade in the lateral parabrachial nucleus

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorSilva, Camila Zambone Cardoso da
dc.creatorMenani, José Vanderlei
dc.creatorCallera, João Carlos
dc.date2013-09-30T18:29:19Z
dc.date2014-05-20T13:43:05Z
dc.date2013-09-30T18:29:19Z
dc.date2014-05-20T13:43:05Z
dc.date2011-11-25
dc.date.accessioned2017-04-05T20:45:59Z
dc.date.available2017-04-05T20:45:59Z
dc.identifierBrain Research Bulletin. Oxford: Pergamon-Elsevier B.V. Ltd, v. 86, n. 5-6, p. 348-354, 2011.
dc.identifier0361-9230
dc.identifierhttp://hdl.handle.net/11449/14998
dc.identifier10.1016/j.brainresbull.2011.09.003
dc.identifierWOS:000298711300008
dc.identifierWOS000298711300008.pdf
dc.identifierhttp://dx.doi.org/10.1016/j.brainresbull.2011.09.003
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/862072
dc.descriptionGABA(A) and GABA(B) receptors activation with agonists muscimol and baclofen, respectively in the lateral parabrachial nucleus (LPBN), induces water and hypertonic NaCl intake in rats. The purpose of this study was to examine the effects of previous injections of losartan (AT(1) angiotensin receptor antagonist) into the LPBN on 0.3 M NaCl and water intake induced by baclofen injected bilaterally in the same area in fluid replete rats and in rats treated with the diuretic furosemide combined with a low dose of the angiotensin-converting enzyme inhibitor captopril injected subcutaneously. Male Wistar rats with stainless steel cannulas implanted bilaterally into the LPBN were used. Bilateral injections of baclofen (0.5 nmol/0.2 mu l, n=6) into the LPBN in fluid replete rats induced 0.3 M NaCl intake (22.4 +/- 6.5 vs. saline: 0.1 +/- 0.1 ml/210 min) and water intake (14.2 +/- 4.0 vs. saline: 0.6 +/- 0.6 ml/210 min) and pre-treatment of the LPBN with losartan (50 mu g/0.2 mu l,l) reduced 0.3 M NaCl intake (7.4 +/- 7.0 ml/210 min) and water intake (2.8 +/- 2.4 ml/210 min) induced by baclofen. In rats treated with furosemide + captopril, pre-treatment with losartan into the LPBN attenuated the increase in 0.3 M NaCl intake (13.3 +/- 3.2 vs. saline + baclofen: 24.3 +/- 3.9 ml/180 min) and water intake (4.8 +/- 2.1 vs. saline + baclofen: 19.5 +/- 6.6 ml/180 min) produced by baclofen. We conclude that baclofen may produce a non-specific blockade of the inhibitory mechanisms of LPBN (deactivation of LPBN inhibitory mechanisms) and this blockade is facilitated by angiotensin II acting on AT(1) receptors in the LPBN, which drives rats to ingest large amounts of water and hypertonic NaCl independent if rats are fluid depleted or normohydrated. (C) 2011 Elsevier B.V. All rights reserved.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageeng
dc.publisherPergamon-Elsevier B.V. Ltd
dc.relationBrain Research Bulletin
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectGABA receptors
dc.subjectAngiotensin II
dc.subjectLosartan
dc.subjectBaclofen
dc.subjectSodium appetite
dc.subjectThirst
dc.subjectLateral parabrachial nucleus
dc.titleNatriorexigenic effect of baclofen is reduced by AT(1) receptor blockade in the lateral parabrachial nucleus
dc.typeOtro


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