dc.contributor | Universidade Federal de São Paulo (UNIFESP) | |
dc.creator | Caseli, Luciano [UNIFESP] | |
dc.creator | Cavalheiro, Renan Pelluzzi [UNIFESP] | |
dc.creator | Nader, Helena Bonciani [UNIFESP] | |
dc.creator | Lopes, Carla Cristina [UNIFESP] | |
dc.date.accessioned | 2016-01-24T14:27:08Z | |
dc.date.accessioned | 2023-09-04T18:33:55Z | |
dc.date.available | 2016-01-24T14:27:08Z | |
dc.date.available | 2023-09-04T18:33:55Z | |
dc.date.created | 2016-01-24T14:27:08Z | |
dc.date.issued | 2012-05-01 | |
dc.identifier | Biochimica Et Biophysica Acta-biomembranes. Amsterdam: Elsevier B.V., v. 1818, n. 5, p. 1211-1217, 2012. | |
dc.identifier | 0005-2736 | |
dc.identifier | http://repositorio.unifesp.br/handle/11600/34810 | |
dc.identifier | WOS000302971100011.pdf | |
dc.identifier | 10.1016/j.bbamem.2012.01.012 | |
dc.identifier | WOS:000302971100011 | |
dc.identifier.uri | https://repositorioslatinoamericanos.uchile.cl/handle/2250/8616229 | |
dc.description.abstract | Investigating the role of proteoglycans associated to cell membranes is fundamental to comprehend biochemical process that occurs at the level of membrane surfaces. in this paper, we exploit syndecan-4, a heparan sulfate proteoglycan obtained from cell cultures, in lipid Langmuir monolayers at the air-water interface. the monolayer served as a model for half a membrane, and the molecular interactions involved could be evaluated with tensiometry and vibrational spectroscopy techniques. Polarization-modulation infrared reflection-absorption spectroscopy (PM-IRRAS) employed in a constant surface pressure regime showed that the main chemical groups for syndecan-4 were present at the air-water interface. Subsequent monolayer decompression and compression showed surface pressure-area isotherms with a large expansion for the lipid monolayers interacting with the cell culture reported to over-express syndecan-4, which was also an indication that the proteoglycan was inserted in the lipid monolayer. the introduction of biological molecules with affinity for syndecam-4, such as growth factors, which present a key role in biochemical process of cell signaling, changed the surface properties of the hybrid film, leading to a model, by which the growth factor binds to the sulfate groups present in the heparan sulfate chains. the polypeptide moiety of syndecan-4 responds to this interaction changing its conformation, which leads to lipid film relaxation and further monolayer condensation. (C) 2012 Elsevier B.V. All rights reserved. | |
dc.language | eng | |
dc.publisher | Elsevier B.V. | |
dc.relation | Biochimica Et Biophysica Acta-biomembranes | |
dc.rights | http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy | |
dc.rights | Acesso aberto | |
dc.subject | Heparan sulfate proteoglycan | |
dc.subject | Syndecan-4 | |
dc.subject | Molecular recognizing | |
dc.subject | Langmuir monolayer | |
dc.title | Probing the interaction between heparan sulfate proteoglycan with biologically relevant molecules in mimetic models for cell membranes: A Langmuir film study | |
dc.type | Artigo | |