dc.contributorDrexel Univ
dc.contributorUniversidade Federal de São Paulo (UNIFESP)
dc.contributorNagoya City Univ Hosp
dc.creatorMedalha, Carla Christina [UNIFESP]
dc.creatorJin, Ying
dc.creatorYamagami, Takaya
dc.creatorHaas, Christopher
dc.creatorFischer, Itzhak
dc.date.accessioned2016-01-24T14:37:13Z
dc.date.accessioned2023-09-04T18:30:52Z
dc.date.available2016-01-24T14:37:13Z
dc.date.available2023-09-04T18:30:52Z
dc.date.created2016-01-24T14:37:13Z
dc.date.issued2014-05-01
dc.identifierJournal of Neuroscience Research. Hoboken: Wiley-Blackwell, v. 92, n. 5, p. 607-618, 2014.
dc.identifier0360-4012
dc.identifierhttp://repositorio.unifesp.br/handle/11600/37709
dc.identifier10.1002/jnr.23340
dc.identifierWOS:000332553400007
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8615591
dc.description.abstractNeural progenitor cell (NPC) transplantation is a promising therapeutic strategy for spinal cord injury (SCI) because of the potential for cell replacement and restoration of connectivity. Our previous studies have shown that transplants of NPC, composed of neuron- and glia-restricted progenitors derived from the embryonic spinal cord, survived well in partial lesion models and generated graft-derived neurons, which could be used to form a functional relay. We have now examined the properties of a similar NPC transplant using a complete transection model in juvenile and adult rats. We found poor survival of grafted cells despite using a variety of lesion methods, matrices, and delays of transplantation. If, instead of cultured progenitor cells, the transplants were composed of segmental or dissociated segments of fetal spinal cord (FSC) derived from similar-staged embryos, grafted cells survived and integrated well with host tissue in juvenile and adult rats. FSC transplants differentiated into neurons and glial cells, including astrocytes and oligodendrocytes. Graft-derived neurons expressed glutaminergic and GABAergic markers. Grafted cells also migrated and extended processes into host tissue. Analysis of axon growth from the host spinal cord showed serotonin-positive fibers and biotinylated dextran amine-traced propriospinal axons growing into the transplants. These results suggest that in treating severe SCI, such as complete transection, NPC grafting faces major challenges related to cell survival and formation of a functional relay. Lessons learned from the efficacy of FSC transplants could be used to develop a therapeutic strategy based on neural progenitor cells for severe SCI. (c) 2014 Wiley Periodicals, Inc.
dc.languageeng
dc.publisherWiley-Blackwell
dc.relationJournal of Neuroscience Research
dc.rightshttp://olabout.wiley.com/WileyCDA/Section/id-406071.html
dc.rightsAcesso restrito
dc.subjectspinal cord injury
dc.subjectcell transplantation
dc.subjectaxonal growth
dc.subjectfetal spinal cord
dc.subjectneural progenitor cells
dc.titleTransplanting neural progenitors into a complete transection model of spinal cord injury
dc.typeArtigo


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