dc.contributorUniversidade Federal de São Paulo (UNIFESP)
dc.contributorSão Paulo State Univ
dc.creatorCorrêa, Silvana Aparecida Alves [UNIFESP]
dc.creatorPacheco, NAS
dc.creatorCosta-Neto, C. M.
dc.creatorOliveira, L.
dc.creatorPesquero, J. B.
dc.creatorHan, S. W.
dc.creatorPaiva, ACM
dc.creatorShimuta, S. I.
dc.date.accessioned2016-01-24T12:38:10Z
dc.date.accessioned2023-09-04T18:23:10Z
dc.date.available2016-01-24T12:38:10Z
dc.date.available2023-09-04T18:23:10Z
dc.date.created2016-01-24T12:38:10Z
dc.date.issued2005-11-15
dc.identifierRegulatory Peptides. Amsterdam: Elsevier B.V., v. 131, n. 1-3, p. 18-22, 2005.
dc.identifier0167-0115
dc.identifierhttp://repositorio.unifesp.br/handle/11600/28551
dc.identifier10.1016/j.regpep.2005.05.005
dc.identifierWOS:000232709100003
dc.identifier.urihttps://repositorioslatinoamericanos.uchile.cl/handle/2250/8613970
dc.description.abstractTo assess the importance of the leucine residues in positions 262 and 265 of the angiotensin AT, receptor for signaling pathways and receptor expression and regulation, we compared the properties of CHO cells transfected with the wild type or the L262D or L265D receptor point mutants. It was found that the two mutants significantly increased the basal intracellular cyclic AMP (cAMP) formation in an agonist-independent mode. the morphology transformation of CHO cells was correlated with the increased cAMP formation, since forskolin, a direct activator of adenylate cyclase mimicked this effect on WT-expressing CHO cells. DNA synthesis was found to be inhibited in these cell lines, indicating that cAMP may also have determined the inhibitory effect on cell growth, in addition to the cell transformation from a tumorigenic to a non-tumorigenic phenotype. However a role for an increased Ca2(+) influx induced by the mutants in non-stimulated cells cannot be ruled out since this ion also was shown to cause transformed cells to regain the morphology and growth regulation. (c) 2005 Elsevier B.V. All rights reserved.
dc.languageeng
dc.publisherElsevier B.V.
dc.relationRegulatory Peptides
dc.rightshttp://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.rightsAcesso restrito
dc.subjectAT(1) receptor
dc.subjectmutagenesis
dc.subjectcyclic AMP
dc.subjectCa+2 signaling
dc.subjectcell proliferation
dc.subjectmorphology regulation
dc.titleAngiotensin II AT(1) receptor mutants expressed in CHO cells caused morphological change and inhibition of cell growth
dc.typeArtigo


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