Independent clonal origin of multiple uterine leiomyomas that was determined by X chromosome inactivation and microsatellite analysis

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorCanevari, R. A.
dc.creatorPontes, Anaglória
dc.creatorRosa, F. E.
dc.creatorRainho, C. A.
dc.creatorRogatto, Silvia Regina
dc.date2014-05-20T13:38:44Z
dc.date2016-10-25T16:54:49Z
dc.date2014-05-20T13:38:44Z
dc.date2016-10-25T16:54:49Z
dc.date2005-10-01
dc.date.accessioned2017-04-05T20:35:51Z
dc.date.available2017-04-05T20:35:51Z
dc.identifierAmerican Journal of Obstetrics and Gynecology. St Louis: Mosby, Inc., v. 193, n. 4, p. 1395-1403, 2005.
dc.identifier0002-9378
dc.identifierhttp://hdl.handle.net/11449/13425
dc.identifierhttp://acervodigital.unesp.br/handle/11449/13425
dc.identifier10.1016/j.ajog.2005.02.097
dc.identifierWOS:000232408000017
dc.identifierhttp://dx.doi.org/10.1016/j.ajog.2005.02.097
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/860761
dc.descriptionObjective: In an attempt to clarify the clonality and genetic relationships that are involved in the tumorigenesis of uterine leiomyomas, we used a total of 43 multiple leiomyomas from 14 patients and analyzed the allelic status with 15 microsatellite markers and X chromosome inactivation analysis.Study design: We have used a set of 15 microsatellite polymorphism markers mapped on 3q, 7p, 11, and 15q by automated analysis. The X chromosome inactivation was evaluated by the methylation status of the X-linked androgen receptor gene.Results: Loss of heterozygosity analysis showed a different pattern in 7 of the 8 cases with allelic loss for at least 1 of 15 microsatellite markers that were analyzed. A similar loss of heterozygosity findings at 7p22-15 was detected in 3 samples from the same patient. X chromosome inactivation analysis demonstrated the same inactivated allele in all tumors of the 9 of 12 informative patients;. different inactivation patterns were observed in 3 cases.Conclusion: Our data support the concept that uterine leiomyomas are derived from a single cell but are generated independently in the uterus. Loss of heterozygosity findings at 7p22-15 are consistent with previous data that suggested the relevance of chromosomal aberrations at 7p that were involved in individual uterine leiomyomas. (C) 2005 Mosby, Inc. All rights reserved.
dc.languageeng
dc.publisherMosby, Inc
dc.relationAmerican Journal of Obstetrics and Gynecology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectloss of heterozygosity
dc.subjectX chromosome inactivation
dc.subjectclonality
dc.subjectuterine leiomyoma
dc.titleIndependent clonal origin of multiple uterine leiomyomas that was determined by X chromosome inactivation and microsatellite analysis
dc.typeOtro


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