Loss of samples in the tissue microarray technique: comparison between slides using adhesive tape and silanized slides

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorLima, Flávio de Oliveira
dc.creatorAlmeida, Joaquim Soares de
dc.creatorCosta, Henrique de Oliveira
dc.creatorPinheiro, Nathanael F.
dc.creatorOshima, Celina Tizuko
dc.creatorFranco, Marcello F. de
dc.date2014-05-20T13:37:35Z
dc.date2016-10-25T16:54:14Z
dc.date2014-05-20T13:37:35Z
dc.date2016-10-25T16:54:14Z
dc.date2011-06-01
dc.date.accessioned2017-04-05T20:33:46Z
dc.date.available2017-04-05T20:33:46Z
dc.identifierJournal of Histotechnology. Leeds: Maney Publishing, v. 34, n. 2, p. 69-73, 2011.
dc.identifier0147-8885
dc.identifierhttp://hdl.handle.net/11449/13027
dc.identifierhttp://acervodigital.unesp.br/handle/11449/13027
dc.identifier10.1179/2046023611Y.0000000001
dc.identifierWOS:000293082000003
dc.identifierhttp://dx.doi.org/10.1179/2046023611Y.0000000001
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/860492
dc.descriptionThe tissue microarray (TMA) technique allows multiple tissue samples in a single block. Commercial adhesive tape is used to avoid the loss of tissue samples during the immunostaining process. Few reports exist in the literature comparing the use of these adhesive tapes to other adhesive techniques. The objective of this study was to compare loss of sections adhered to slides using commercial adhesive tapes versus using silanized only slides. TMA was constructed with varying tissues using a fixed-base device (Beecher Instruments), placing 108 cylinders of 1 mm diameter in duplicate, spaced 1.2 mm apart. Section of 4 mu m were cut from the TMA block and adhered to 30 silanized slides and 30 commercial glass slides using adhesive tape, according to manufacturer's recommendations. Vimentin immunoexpression was evaluated by immunohistochemistry. Antigenic recovery was realized in citrate buffer using a microwave oven. Cylinder loss in the immunohistochemical process was quantified and expressed as: total (>80%), almost complete (75-79%), or partial (50-74%). The commercial adhesive tape group presented lesser total loss (1.1 versus 6.4%), almost complete loss (2.2 versus 3.5%), and partial loss (2.1 versus 3.8%) than the silanized slide group (ANOVA, P < 0.05). The sum of total and almost complete losses in the silanized slide group was 9.9%, greater than the losses in slides using commercial adhesive tapes (3.3%) and less than reported and considered acceptable in the literature (10-30%). In conclusion, the use of silanized only slides presents very satisfactory results, requires less training, and reduces costs significantly, thus justifying their use in research.
dc.languageeng
dc.publisherManey Publishing
dc.relationJournal of Histotechnology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectImmunohistochemistry
dc.subjectSample losses
dc.subjectTissue microarray
dc.titleLoss of samples in the tissue microarray technique: comparison between slides using adhesive tape and silanized slides
dc.typeOtro


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