Genotoxicity in primary human peripheral lymphocytes after exposure to radiopacifiers in vitro

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorBraz, Mariana Gobbo
dc.creatorMarcondes, Joao Paulo de Castro
dc.creatorMatsumoto, Mariza Akemi
dc.creatorDuarte, Marco Antonio Hungaro
dc.creatorSalvadori, Daisy Maria Favero
dc.creatorRibeiro, Daniel Araki
dc.date2014-05-20T13:37:31Z
dc.date2016-10-25T16:54:11Z
dc.date2014-05-20T13:37:31Z
dc.date2016-10-25T16:54:11Z
dc.date2008-02-01
dc.date.accessioned2017-04-05T20:33:34Z
dc.date.available2017-04-05T20:33:34Z
dc.identifierJournal of Materials Science-materials In Medicine. Dordrecht: Springer, v. 19, n. 2, p. 601-605, 2008.
dc.identifier0957-4530
dc.identifierhttp://hdl.handle.net/11449/12991
dc.identifierhttp://acervodigital.unesp.br/handle/11449/12991
dc.identifier10.1007/s10856-007-3000-2
dc.identifierWOS:000252976100016
dc.identifier0000-0001-5389-0105
dc.identifier0000-0003-4413-226X
dc.identifierhttp://dx.doi.org/10.1007/s10856-007-3000-2
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/860463
dc.descriptionTaking into consideration that DNA damage plays an important role in carcinogenesis, the purpose of this study was to evaluate whether some radiopacifiers widely used in clinical practice are able to induce genetic damage in primary human cells in vitro. Human peripheral lymphocytes obtained from 10 healthy volunteers were exposed to barium sulphate (BaSO(4)), zirconium oxide (ZnO(2)) and bismuth oxide (Bi(2)O(3)) at final concentrations ranging from 1 to 1000 mu g/mL for 1 h at 37 degrees C. The negative control group was treated with vehicle control (phosphate buffer solution) for 1 h at 37 degrees C and the positive control group was treated with hydrogen peroxide (at 100 mu M) for 5 min on ice. Results were analyzed by the Friedman non-parametric test. The results pointed all compounds tested out did not induce DNA breakage in human peripheral lymphocytes as depicted by the mean tail moment and tail intensity in all concentrations tested. In summary, our results indicate that exposure to these radiopacifiers may not be a factor that increases the level of DNA lesions in human peripheral lymphocytes as detected by single cell gel (comet) assay.
dc.languageeng
dc.publisherSpringer
dc.relationJournal of Materials Science: Materials in Medicine
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.titleGenotoxicity in primary human peripheral lymphocytes after exposure to radiopacifiers in vitro
dc.typeOtro


Este ítem pertenece a la siguiente institución