Absence of DNA damage in multiple organs (blood, liver, kidney, thyroid gland and urinary bladder) after acute fluoride exposure in rats

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorLeite, Aline de Lima
dc.creatorSantiago, Joel Ferreira
dc.creatorLevy, Flavia Mauad
dc.creatorMaria, Andrea Gutierrez
dc.creatorFernandes, Mileni da Silva
dc.creatorSalvadori, Daisy Maria Favero
dc.creatorRibeiro, Daniel Araki
dc.creatorRabelo Buzalaf, Marflia Afonso
dc.date2014-05-20T13:37:06Z
dc.date2016-10-25T16:53:56Z
dc.date2014-05-20T13:37:06Z
dc.date2016-10-25T16:53:56Z
dc.date2007-05-01
dc.date.accessioned2017-04-05T20:32:40Z
dc.date.available2017-04-05T20:32:40Z
dc.identifierHuman & Experimental Toxicology. London: Sage Publications Ltd, v. 26, n. 5, p. 435-440, 2007.
dc.identifier0960-3271
dc.identifierhttp://hdl.handle.net/11449/12817
dc.identifierhttp://acervodigital.unesp.br/handle/11449/12817
dc.identifier10.1177/0960327107076288
dc.identifierWOS:000248156000006
dc.identifierhttp://dx.doi.org/10.1177/0960327107076288
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/860351
dc.descriptionFluoride has been widely used in dentistry as a caries prophylactic agent. However, there has been some speculation that excess fluoride could cause an impact on genome integrity. In the current study, the potential DNA damage associated with exposure to fluoride was assessed in cells of blood, liver, kidney, thyroid gland and urinary bladder by the single cell gel (comet) assay. Male Wistar rats aging 75 days were distributed into seven groups: Groups 1 (control), 2, 3, 4, 5, 6 and 7 received 0 (deionized water), 10, 20, 40, 60, 80 and 100 mgF/Kg body weight from sodium fluoride (NaF), respectively, by gastrogavage. These groups were killed at 2 h after the administration of the fluoride doses. The level of DNA strand breaks did not increase in all organs evaluated and at all doses of NaF tested, as depicted by the mean tail moment. Taken together, our results suggest that oral exposure to NaF did not result in systemic genotoxic effect in multiple organs related to fluoride toxicity. Since DNA damage is an important step in events leading to carcinogenesis, this study represents a relevant contribution to the correct evaluation of the potential health risk associated with chemical exposure.
dc.languageeng
dc.publisherSage Publications Ltd
dc.relationHuman & Experimental Toxicology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectacute exposure
dc.subjectcomet assay
dc.subjectDNA damage
dc.subjectfluoride
dc.subjectrats
dc.titleAbsence of DNA damage in multiple organs (blood, liver, kidney, thyroid gland and urinary bladder) after acute fluoride exposure in rats
dc.typeOtro


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