dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorPinhal, D.
dc.creatorGontijo, AMDC
dc.creatorReyes, VAV
dc.creatorSalvadori, Daisy Maria Favero
dc.date2014-05-20T13:36:55Z
dc.date2016-10-25T16:53:46Z
dc.date2014-05-20T13:36:55Z
dc.date2016-10-25T16:53:46Z
dc.date2006-03-01
dc.date.accessioned2017-04-05T20:32:02Z
dc.date.available2017-04-05T20:32:02Z
dc.identifierEnvironmental and Molecular Mutagenesis. Hoboken: Wiley-liss, v. 47, n. 2, p. 117-126, 2006.
dc.identifier0893-6692
dc.identifierhttp://hdl.handle.net/11449/12711
dc.identifierhttp://acervodigital.unesp.br/handle/11449/12711
dc.identifier10.1002/em.20174
dc.identifierWOS:000235348400005
dc.identifierhttp://dx.doi.org/10.1002/em.20174
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/860272
dc.descriptionBuccal mucosa (BM) cells have been used in human biomonitoring studies for detecting DNA adducts and chromosomal damage in an epithelial cell population. In the present study, we have investigated if human BM cells are suitable for use in the single-cell gel electrophoresis (SCGE)/Comet assay as an approach for estimating the exposure of epithelial cells to DNA-damaging agents. Our results indicate that only a few cells from BM cell samples yield comets that can be analyzed by current methods, and that the yield of cells with comets is independent of the percentage of viable BM cells in the sample. Data generated after enzymatic enrichment of viable cells and immunomagnetic separation of epithelial cells suggest that most of the BM cells that do form comets are probably leukocytes. Moreover, by reevaluating specific cells after running the Comet assay, we found that viable epithelial BM cells give rise to atypical comets that are not included in the analysis. Comparing DNA migration patterns between small groups of smokers and nonsmokers indicated that long-term smoking had no effect on the subpopulation of cells that yield typical comets. Our results indicate that the SCGE assay, as it is commonly performed, may not be useful for genotoxicity monitoring in human epithelial BM cells.
dc.languageeng
dc.publisherWiley-Blackwell
dc.relationEnvironmental and Molecular Mutagenesis
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectsingle-cell gel electrophoresis assay
dc.subjectDNA damage
dc.subjectviability
dc.subjectsmoking
dc.subjectgenotoxicity
dc.subjectcytotoxicity
dc.titleViable human buccal mucosa cells do not yield typical nucleoids: Impacts on the single-cell gel electrophoresis/comet assay
dc.typeOtro


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