Definition of a diagnostic routine in individuals with inconclusive serology for chagas disease

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorPicka, Mariele Cristina Modolo
dc.creatorMeira, Domingos Alves
dc.creatorCarvalho, Thaís Batista de
dc.creatorPeresi, Eliana
dc.creatorMarcondes-Machado, Jussara
dc.date2014-05-20T13:34:40Z
dc.date2014-05-20T13:34:40Z
dc.date2007-04-01
dc.date.accessioned2017-04-05T20:26:49Z
dc.date.available2017-04-05T20:26:49Z
dc.identifierBrazilian Journal of Infectious Diseases. Brazilian Society of Infectious Diseases, v. 11, n. 2, p. 226-233, 2007.
dc.identifier1413-8670
dc.identifierhttp://hdl.handle.net/11449/11903
dc.identifier10.1590/S1413-86702007000200012
dc.identifierS1413-86702007000200012
dc.identifierS1413-86702007000200012.pdf
dc.identifierhttp://dx.doi.org/10.1590/S1413-86702007000200012
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/859601
dc.descriptionDespite the existence of highly sensitive tests, inconclusive serological results are frequent in chronic chagasic infection. This study aimed to define a diagnostic conduct for 30 individuals with inconclusive serology (G3) for chagasic infection assisted at the Outpatient Unit for Infectious and Parasitic Diseases of the Botucatu School of Medicine. Twenty-one individuals with negative serology (G1) and 33 with positive serology (G2) were also studied. Serological methods ELISA, HAI, IFI and immunoblotting TESA-cruzi were used for G1, G2 and G3, and parasitological methods xenodiagnosis, hemoculture and PCR-LIT were used for G2 and G3 individuals. ELISA, HAI and IFI were performed in 5 different blood samples in G2 and G3. TESA-cruzi was carried out only once in G1, G2 and G3 and, since it is the most sensitive, it was utilized as standard. In G3, positivity for ELISA reached 86% in the fifth blood sample; the ELISA+HAI+IFI combination showed a maximum of 44.8% in the second sample; and TESA-cruzi, 76% in one single sample. Xenodiagnosis positivity was 9.4%; hemoculture showed 15.2%; and PCR-LIT exhibited 22% positivity in G2. Nevertheless, in G3, positivity percentage was 3.4% for xenodiagnosis, 6.7% for PCR-LIT, and no positive result was found for hemoculture. In G3, PCR-LIT resolved one case which was still inconclusive according to serology tests. In order to define inconclusive diagnoses, the results suggest the combined use of ELISA+HAI+IFI in 2 blood samples, decreasing the occurrence of false positive/negative results. If results remain inconclusive, the performance of TESA-cruzi and PCR-LIT, if necessary, is recommended.
dc.languageeng
dc.publisherBrazilian Society of Infectious Diseases
dc.relationBrazilian Journal of Infectious Diseases
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectChronic chagasic infection
dc.subjectTrypanosoma cruzi
dc.subjectinconclusive serology
dc.subjectimmunoblotting
dc.titleDefinition of a diagnostic routine in individuals with inconclusive serology for chagas disease
dc.typeOtro


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