Interleukin-6 treatment enhances human monocyte permissiveness for Paracoccidioides brasiliensis growth by modulating cytokine production

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorSiqueira, Keila Zaniboni
dc.creatorSoares, Ângela Maria Victoriano de Campos
dc.creatorDias-Melicio, Luciane Alarcao
dc.creatorCalvi, Sueli Aparecida
dc.creatorPeraçoli, Maria Terezinha Serrão
dc.date2014-05-20T13:34:31Z
dc.date2016-10-25T16:52:12Z
dc.date2014-05-20T13:34:31Z
dc.date2016-10-25T16:52:12Z
dc.date2009-01-01
dc.date.accessioned2017-04-05T20:26:25Z
dc.date.available2017-04-05T20:26:25Z
dc.identifierMedical Mycology. Abingdon: Taylor & Francis Ltd, v. 47, n. 3, p. 259-267, 2009.
dc.identifier1369-3786
dc.identifierhttp://hdl.handle.net/11449/11845
dc.identifierhttp://acervodigital.unesp.br/handle/11449/11845
dc.identifier10.1080/13693780802244204
dc.identifierWOS:000265367200004
dc.identifierhttp://dx.doi.org/10.1080/13693780802244204
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/859546
dc.descriptionThe effect of interleukin (IL)-6 on cytokine production was evaluated in human monocyte cultures infected with the virulent strain of Paracoccidioides brasiliensis (Pb18). Peripheral blood monocytes from healthy individuals were preincubated for 24 h with or without human recombinant IL-6, and then challenged with Pb18 for 4 h and 18 h. P. brasiliensis growth was assessed by viable fungi recovery from co-cultures after plating on BHI-agar. Moreover, tumor necrosis factor-alpha (TNF-), IL-6 and IL-10 production in supernatant cultures was determined by enzyme immunoassay (ELISA). Monocyte preincubation with IL-6, and challenged with Pb18 for 4 h, led to significantly higher fungi recovery compared to non-treated co-cultures. The pretreatment of monocytes with IL-6 induced an inhibitory effect on TNF- and IL-10 production during 18 h fungal infection. Otherwise, an autocrine stimulatory effect on IL-6 production was detected at 4 h and 18 h as represented by an elevation in IL-6 levels. The reduction in TNF- levels and stimulation of IL-6 production induced by previous IL-6 treatment might be responsible for a significant increase in fungal growth in human monocytes. The results suggest that IL-6, by exerting a modulatory effect on cytokines production, makes monocyte more permissive for fungal growth.
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageeng
dc.publisherTaylor & Francis Ltd
dc.relationMedical Mycology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectinterleukin-6
dc.subjectinterleukin-10
dc.subjectmonocytes
dc.subjectParacoccidioides brasiliensis
dc.subjecttumor necrosis factor-alpha
dc.titleInterleukin-6 treatment enhances human monocyte permissiveness for Paracoccidioides brasiliensis growth by modulating cytokine production
dc.typeOtro


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