DNA damage and nitric oxide production in mice following infection with L. chagasi

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorCardoso de Oliveira, Larissa Ragozo
dc.creatorGomes Cezario, Glaucia Aparecida
dc.creatorGoncalves de Lima, Carlos Roberto
dc.creatorNicolete, Vanessa Cristina
dc.creatorPeresi, Eliana
dc.creatorde Sibio, Maria Tereza
dc.creatorModolo Picka, Mariele Cristina
dc.creatorCalvi, Sueli Aparecida
dc.date2014-05-20T13:34:29Z
dc.date2016-10-25T16:52:10Z
dc.date2014-05-20T13:34:29Z
dc.date2016-10-25T16:52:10Z
dc.date2011-08-16
dc.date.accessioned2017-04-05T20:26:18Z
dc.date.available2017-04-05T20:26:18Z
dc.identifierMutation Research-genetic Toxicology and Environmental Mutagenesis. Amsterdam: Elsevier B.V., v. 723, n. 2, p. 177-181, 2011.
dc.identifier1383-5718
dc.identifierhttp://hdl.handle.net/11449/11827
dc.identifierhttp://acervodigital.unesp.br/handle/11449/11827
dc.identifier10.1016/j.mrgentox.2011.04.009
dc.identifierWOS:000293315400016
dc.identifierhttp://dx.doi.org/10.1016/j.mrgentox.2011.04.009
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/859531
dc.descriptionLeishmania chagasi, which causes visceral leishmaniasis in South America, is an obligate intracellular protozoan. Production of nitric oxide by macrophages during the inflammatory response is one of the main microbicidal mechanisms against this parasite. The goal of this study was to evaluate whether L. chagasi infection causes DNA damage in peripheral blood and spleen cells of Balb/c mice and whether such damage may be related to NO production. Balb/c mice were either infected with L chagasi or maintained as controls. The single-cell gel electrophoresis (comet) assay was used to measure DNA damage in peripheral blood and spleen cells, and the Griess reaction was used to measure NO production in the spleen. L chagasi infection induced DNA damage in peripheral blood and spleen cells of infected mice. Macrophages from the control group, challenged with L. chagasi, showed significantly (p < 0.05) greater NO production, compared to non-challenged cells. Treatment of spleen cells with N(G)-monomethyl-L-arginine (LNMMA) caused a significant reduction of NO production and DNA damage (p < 0.05). Our results indicate that L. chagasi induces DNA damage in the peripheral blood and spleen cells and that NO not only causes killing of the parasite but also induces DNA damage in adjacent cells. (C) 2011 Elsevier B.V. All rights reserved.
dc.languageeng
dc.publisherElsevier B.V.
dc.relationMutation Research: Genetic Toxicology and Environmental Mutagenesis
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectLeishmania chagasi
dc.subjectDNA damage
dc.subjectNO production
dc.titleDNA damage and nitric oxide production in mice following infection with L. chagasi
dc.typeOtro


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