Exercise Training and Caloric Restriction Prevent Reduction in Cardiac Ca2+-Handling Protein Profile in Obese Rats

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorPaulino, Ellena Christina
dc.creatorBatista Ferreira, Julio Cesar
dc.creatorBechara, Luiz Roberto
dc.creatorTsutsui, Jeane Mike
dc.creatorMathias, Wilson
dc.creatorLima, Fabio Bessa
dc.creatorCasarini, Dulce Elena
dc.creatorCicogna, Antonio Carlos
dc.creatorBrum, Patricia Chakur
dc.creatorNegrao, Carlos Eduardo
dc.date2014-05-20T13:33:21Z
dc.date2016-10-25T16:51:26Z
dc.date2014-05-20T13:33:21Z
dc.date2016-10-25T16:51:26Z
dc.date2010-10-01
dc.date.accessioned2017-04-05T20:23:38Z
dc.date.available2017-04-05T20:23:38Z
dc.identifierHypertension. Philadelphia: Lippincott Williams & Wilkins, v. 56, n. 4, p. 629-U137, 2010.
dc.identifier0194-911X
dc.identifierhttp://hdl.handle.net/11449/11413
dc.identifierhttp://acervodigital.unesp.br/handle/11449/11413
dc.identifier10.1161/HYPERTENSIONAHA.110.156141
dc.identifierWOS:000281881400015
dc.identifierhttp://dx.doi.org/10.1161/HYPERTENSIONAHA.110.156141
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/859183
dc.descriptionPrevious studies show that exercise training and caloric restriction improve cardiac function in obesity. However, the molecular mechanisms underlying this effect on cardiac function remain unknown. Thus, we studied the effect of exercise training and/or caloric restriction on cardiac function and Ca2+ handling protein expression in obese rats. To accomplish this goal, male rats fed with a high-fat and sucrose diet for 25 weeks were randomly assigned into 4 groups: high-fat and sucrose diet, high-fat and sucrose diet and exercise training, caloric restriction, and exercise training and caloric restriction. An additional lean group was studied. The study was conducted for 10 weeks. Cardiac function was evaluated by echocardiography and Ca2+ handling protein expression by Western blotting. Our results showed that visceral fat mass, circulating leptin, epinephrine, and norepinephrine levels were higher in rats on the high-fat and sucrose diet compared with the lean rats. Cardiac nitrate levels, reduced/oxidized glutathione, left ventricular fractional shortening, and protein expression of phosphorylated Ser(2808)-ryanodine receptor and Thr(17-)phospholamban were lower in rats on the high-fat and sucrose diet compared with lean rats. Exercise training and/or caloric restriction prevented increases in visceral fat mass, circulating leptin, epinephrine, and norepinephrine levels and prevented reduction in cardiac nitrate levels and reduced: oxidized glutathione ratio. Exercise training and/or caloric restriction prevented reduction in left ventricular fractional shortening and in phosphorylation of the Ser(2808)-ryanodine receptor and Thr(17)-phospholamban. These findings show that exercise training and/or caloric restriction prevent cardiac dysfunction in high-fat and sucrose diet rats, which seems to be attributed to decreased circulating neurohormone levels. In addition, this nonpharmacological paradigm prevents a reduction in the Ser(2808)-ryanodine receptor and Thr(17-)phospholamban phosphorylation and redox status. (Hypertension. 2010;56:629-635.)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageeng
dc.publisherLippincott Williams & Wilkins
dc.relationHypertension
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectlist
dc.subjectobesity
dc.subjectexercise training
dc.subjectcaloric restriction
dc.subjectcardiac dysfunction
dc.subjectCa2+ handling
dc.titleExercise Training and Caloric Restriction Prevent Reduction in Cardiac Ca2+-Handling Protein Profile in Obese Rats
dc.typeOtro


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