Trypanocidal action of eupomatenoid-5 is related to mitochondrion dysfunction and oxidative damage in Trypanosoma cruzi

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorPelizzaro-Rocha, Karin Juliane
dc.creatorVeiga-Santos, Phercyles
dc.creatorLazarin-Bidoia, Danielle
dc.creatorUeda-Nakamura, Tania
dc.creatorDias Filho, Benedito Prado
dc.creatorXimenes, Valdecir Farias
dc.creatorSilva, Sueli Oliveira
dc.creatorNakamura, Celso Vataru
dc.date2014-05-20T13:27:00Z
dc.date2014-05-20T13:27:00Z
dc.date2011-11-01
dc.date.accessioned2017-04-05T20:07:12Z
dc.date.available2017-04-05T20:07:12Z
dc.identifierMicrobes and Infection. Amsterdam: Elsevier B.V., v. 13, n. 12-13, p. 1018-1024, 2011.
dc.identifier1286-4579
dc.identifierhttp://hdl.handle.net/11449/8789
dc.identifier10.1016/j.micinf.2011.05.011
dc.identifierWOS:000296216000008
dc.identifierWOS000296216000008.pdf
dc.identifierhttp://dx.doi.org/10.1016/j.micinf.2011.05.011
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/857080
dc.descriptionBecause of its severe side effects and variable efficacy, the current treatment for Chagas disease is unsatisfactory. Natural compounds are good alternative chemotherapeutic agents for the treatment of this infection. Recently, our group reported the antiproliferative activity and morphological alterations in epimastigotes and intracellular amastigotes of Trypanosoma cruzi treated with eupomatenoid-5, a neolignan isolated from leaves of Piper regnellii var. pallescens. Here, we demonstrate that eupomatenoid-5 exhibited activity against trypomastigotes, the infective form of T cruzi (EC(50) 40.5 mu W), leading to ultrastructural alteration and lipoperoxidation in the cell membrane. Additionally, eupomatenoid-5 induced depolarization of the mitochondrial membrane, lipoperoxidation and increased G6PD activity in epimastigotes of T cruzi. These findings support the possibility that different mechanisms may be targeted, according to the form of the parasite, and that the plasma membrane and mitochondria are the structures that are most affected in trypomastigotes and epimastigotes, respectively. Thus, the trypanocidal action of eupomatenoid-5 may be associated with mitochondrial dysfunction and oxidative damage, which can trigger destructive effects on biological molecules of T cruzi, leading to parasite death. (C) 2011 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.languageeng
dc.publisherElsevier B.V.
dc.relationMicrobes and Infection
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectTrypanosoma cruzi
dc.subjectEupomatenoid-5
dc.subjectAntioxidant system
dc.titleTrypanocidal action of eupomatenoid-5 is related to mitochondrion dysfunction and oxidative damage in Trypanosoma cruzi
dc.typeOtro


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