Mycobacterium bovis identification by a molecular method from post-mortem inspected cattle obtained in abattoirs of Mato Grosso do Sul, Brazil

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorAraújo, Cristina Pires de
dc.creatorLeite, Clarice Queico Fujimura
dc.creatorPrince, Karina Andrade de
dc.creatorJorge, Klaudia dos Santos Gonçalves
dc.creatorOsório, Ana Luiza Alves Rosa
dc.date2014-05-20T13:25:21Z
dc.date2014-05-20T13:25:21Z
dc.date2005-11-01
dc.date.accessioned2017-04-05T20:02:51Z
dc.date.available2017-04-05T20:02:51Z
dc.identifierMemórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 100, n. 7, p. 749-752, 2005.
dc.identifier0074-0276
dc.identifierhttp://hdl.handle.net/11449/8019
dc.identifier10.1590/S0074-02762005000700013
dc.identifierS0074-02762005000700013
dc.identifierS0074-02762005000700013.pdf
dc.identifierhttp://dx.doi.org/10.1590/S0074-02762005000700013
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/856520
dc.descriptionThe presence of Mycobacterium bovis in bovine carcasses with lesions suggestive of tuberculosis was evaluated. Seventy-two carcass samples were selected during slaughter inspection procedures in abattoirs in the state of Mato Grosso do Sul, Brazil. Seventeen (23.6%) of samples showed colonies suggestive of mycobacteria that were confirmed to be acid-fast bacilli by Ziehl-Neelsen staining. Polymerase chain reaction (PCR) using primers specific for M. bovis identified M. bovis in 13 (76.5%) isolates. The PCR-restriction enzyme pattern analysis using gene encoding for the 65-kDa protein and two restriction enzymes identified the remaining four isolates that were represented by two M. tuberculosis complex and two nontuberculous mycobacteria. The results are indicative of infection of slaughter cattle by M. bovis and other mycobacteria in the state of Mato Grosso do Sul.
dc.languageeng
dc.publisherInstituto Oswaldo Cruz, Ministério da Saúde
dc.relationMemórias do Instituto Oswaldo Cruz
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectMycobacterium bovis
dc.subjectnontuberculous mycobacteria
dc.subjectpolymerase chain reaction
dc.subjectPCR- restriction enzyme pattern analysis
dc.subjectcattle
dc.subjectpost-mortem inspection
dc.subjectabattoirs
dc.titleMycobacterium bovis identification by a molecular method from post-mortem inspected cattle obtained in abattoirs of Mato Grosso do Sul, Brazil
dc.typeOtro


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