Trypanosoma cruzi: establishment of permeable cells for RNA processing analysis with drugs

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorAmbrósio, DL
dc.creatorBarbosa, CF
dc.creatorVianna, VF
dc.creatorCicarelli, RMB
dc.date2014-05-20T13:25:20Z
dc.date2014-05-20T13:25:20Z
dc.date2004-10-01
dc.date.accessioned2017-04-05T20:02:50Z
dc.date.available2017-04-05T20:02:50Z
dc.identifierMemórias do Instituto Oswaldo Cruz. Instituto Oswaldo Cruz, Ministério da Saúde, v. 99, n. 6, p. 617-620, 2004.
dc.identifier0074-0276
dc.identifierhttp://hdl.handle.net/11449/8017
dc.identifier10.1590/S0074-02762004000600014
dc.identifierS0074-02762004000600014
dc.identifierS0074-02762004000600014.pdf
dc.identifierhttp://dx.doi.org/10.1590/S0074-02762004000600014
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/856518
dc.descriptionPre-mRNA maturation in trypanosomatids occurs through a process called trans-splicing which involves excision of introns and union of exons in two independent transcripts. For the first time, we present the standardization of Trypanosoma cruzi permeable cells (Y strain) as a model for trans-splicing study of mRNAs in trypanosomes, following by RNase protection reaction, which localizes the SL exon and intron. This trans-splicing reaction in vitro was also used to analyze the influence of NFOH-121, a nitrofurazone-derivative, on this mechanism. The results suggested that the prodrug affects the RNA processing in these parasites, but the trans-splicing reaction still occurred.
dc.languageeng
dc.publisherInstituto Oswaldo Cruz, Ministério da Saúde
dc.relationMemórias do Instituto Oswaldo Cruz
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjecttrans-splicing
dc.subjectTrypanosoma cruzi
dc.subjectNFOH-121 (hydroxymethylnitrofurazone)
dc.titleTrypanosoma cruzi: establishment of permeable cells for RNA processing analysis with drugs
dc.typeOtro


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