LC Evaluation of Intestinal Transport of Praziquantel

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorCinto, Priscila O.
dc.creatorRibeiro Souza, Ana Luiza
dc.creatorLima, Andrea C.
dc.creatorChaud, Marco V.
dc.creatorGremião, Maria Palmira Daflon
dc.date2014-05-20T13:24:50Z
dc.date2016-10-25T16:45:29Z
dc.date2014-05-20T13:24:50Z
dc.date2016-10-25T16:45:29Z
dc.date2009-06-01
dc.date.accessioned2017-04-05T20:01:24Z
dc.date.available2017-04-05T20:01:24Z
dc.identifierChromatographia. Wiesbaden: Vieweg, v. 69, p. 213-217, 2009.
dc.identifier0009-5893
dc.identifierhttp://hdl.handle.net/11449/7808
dc.identifierhttp://acervodigital.unesp.br/handle/11449/7808
dc.identifier10.1365/s10337-009-1019-x
dc.identifierWOS:000267330400017
dc.identifierhttp://dx.doi.org/10.1365/s10337-009-1019-x
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/856345
dc.descriptionPraziquantel (PZQ) is a highly lipophilic drug with low aqueous solubility. Despite this, it is well absorbed from the gastrointestinal tract. In this study, a simple LC method was developed and validated, in order to monitor the concentration of PZQ in TC-199 buffer in vitro, in the rat everted gut sac absorption model. PZQ was analyzed by a reversed-phase LC method with an isocratic mobile phase containing acetonitrile and water in the proportions 45:55. The flow-rate was 1 mL min(-1) and PZQ was determined by measuring absorbance at 215 nm, at 25 A degrees C. The method was found to be specific, as none of the components of TC-199 or intestinal sac artefacts interfered with the drug peak. Recovery was within acceptable statistical limits. The limit of detection was 0.54 mu g mL(-1) and the limit of quantitation was 1.63 mu g mL(-1). The calibration curve was found to be linear in the concentration range of 10-90 mu g mL(-1) PZQ. The proposed method was found to be rapid and selective and hence can be applied in the monitoring of the absorption of PZQ in in vitro everted gut sac absorption studies.
dc.descriptionCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.languageeng
dc.publisherVieweg
dc.relationChromatographia
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectColumn liquid chromatography
dc.subjectIntestinal permeation assay
dc.subjectPraziquantel
dc.titleLC Evaluation of Intestinal Transport of Praziquantel
dc.typeOtro


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