First Identification of Culex flavivirus (Flaviviridae) in Brazil

dc.contributorUniversidade Estadual Paulista (UNESP)
dc.creatorMachado, Daiane Cristina
dc.creatorMondini, Adriano
dc.creatorSantana, Vinicius dos Santos
dc.creatorKamiya Yonamine, Patricia Tiemi
dc.creatorChiaravalloti Neto, Francisco
dc.creatorde Andrade Zanotto, Paolo Marinho
dc.creatorNogueira, Mauricio Lacerda
dc.date2014-05-20T13:24:17Z
dc.date2016-10-25T16:45:01Z
dc.date2014-05-20T13:24:17Z
dc.date2016-10-25T16:45:01Z
dc.date2012-01-01
dc.date.accessioned2017-04-05T19:59:40Z
dc.date.available2017-04-05T19:59:40Z
dc.identifierIntervirology. Basel: Karger, v. 55, n. 6, p. 475-483, 2012.
dc.identifier0300-5526
dc.identifierhttp://hdl.handle.net/11449/7494
dc.identifierhttp://acervodigital.unesp.br/handle/11449/7494
dc.identifier10.1159/000337166
dc.identifierWOS:000309519100009
dc.identifierhttp://dx.doi.org/10.1159/000337166
dc.identifier.urihttp://repositorioslatinoamericanos.uchile.cl/handle/2250/856123
dc.descriptionIntroduction: Culex flavivirus (CxFV) was first isolated in 2007 from Culex pipiens in Japan and then identified in several other countries. Characterization of the CxFV showed that all strains are related to the cell fusing agent virus. In this manuscript we report the first identification of CxFV in South America. Material and Methods: We have collected Culex sp. mosquitoes using BG-Sentinel traps and manual aspirators. They were pooled according to genus, species, sex and location. Viral RNA was extracted and multiplex nested PCR was performed to test the presence of Flavivirus. The positive samples were isolated in C6/36 cells and sequenced for phylogenetic analyses. Results: 265 female Culex mosquitoes pooled in 83 pools were tested with specific CxFV, Saint Louis encephalitis virus (SLEV) and West Nile virus (WNV) primers. Our sequence data indicated maximum sequence similarity of 97% with CxFV. Discussion: In this study we report the circulation of CxFV in an urban setting where SLEV had previously caused an outbreak. In terms of public health, this is an important finding due to the assumption that the previous exposition of mosquitoes to CxFV might lessen the susceptibility of these mosquitoes to other flaviviruses. Copyright (C) 2012 S. Karger AG, Basel
dc.descriptionConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
dc.descriptionFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
dc.languageeng
dc.publisherKarger
dc.relationIntervirology
dc.rightsinfo:eu-repo/semantics/closedAccess
dc.subjectCulex flavivirus
dc.subjectWest Nile virus
dc.subjectPhylogenetic analysis
dc.titleFirst Identification of Culex flavivirus (Flaviviridae) in Brazil
dc.typeOtro


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